Hydrolysates from cauliflower and artichoke industrial wastes as biostimulants on seed germination and seedling growth. A chemical and biological characterization

Background: Cauliflower (Brassica oleracea L.) and globe artichoke (Cynara scolymus L.) are vegetables with a high waste index mainly related to stems and leaves. In this study, enzymatic hydrolysates obtained from these wastes were proposed to be usedas plant biostimulants. Life cycle assessment methodology was also applied to evaluate environmental performances related tocauliflower and artichoke byproducts.Results: Hydrolysates (HYs) were chemically and biologically characterized. Amino acids, organic acids, amines, polyols, mineral ele-ments, phenols, tannins, flavonoids and sulfur compounds were identified and quantified by means of NMR, inductively coupled plasma mass spectrometry and UV–visible analyses. Cauliflower leaf and flower HYs showed the highest concentration of free aminoacids, whereas stems showed the highest concentration of Ca. Regarding artichoke, asparagine, glutamine and aspartic acid wereexclusively detected in stems, whereas artichoke leaves showed the highest Mg and Mn levels together with the highest antioxidantactivity. The HYs diluted in water were tested as biostimulants. The impacts of five concentrations of HYs (0.00, 0.28, 0.84, 2.52 and7.56 g L−1) on seed germination and early seedling growth of crimson clover, alfalfa, durum wheat and corn were investigated.Conclusions: The application of artichoke biostimulant (0.28 g L−1) positively influenced the coefficient of velocity of germination in alfalfa, crimson clover and durum wheat, whereas cauliflower biostimulant significantly improved corn germination speed.