Advanced drug delivery systems have been developed to overcome the major drawbacks associated with the conventional ones. The aim of our work is to develop nanoemulsions suitable for delivering bioactive substances. Nanoemulsions (NEs) are nano-sized emulsions, manufactured for improving the delivery of active pharmaceutical ingredients. These carriers are composed by oil and surfactants; in particular, natural oils can exhibit anti-inflammatory, antioxidant, antibacterial, antiviral, anticancer and/or tissue regenerative activity, due to the presence of polyphenols and tocopherols. The droplet size of NEs falls typically in the range 10–200 nm. They are kinetically more stable than microemulsions with no apparent flocculation or coalescence; they also improve intracellular penetration in biological tissues, bioavailability, tolerability and solubility of lipophilic drugs. As a result of the slow release of active compounds, the irritability described in tissues is low. NEs are also non-toxic and can be formulated for different applications, such as topical, ocular, intravenous, intranasal and oral delivery. In this study, nanoemulsions from Neem seed oil and Tween 20 or Tween 80 as non-ionic surfactants were prepared. In particular, Neem oil was selected since it shows many bioactive properties, such as antibacterial, antimalarial and antifungal activity. A mean droplet size ranging from 10–100 nm was obtained by modulating the oil/surfactant ratio. Several nanoemulsion formulations were characterized in terms of size and ζ-potential and physicochemical properties, such as microviscosity, polarity and turbidity, were evaluated. Furthermore stability studies were carried out for a period of 60 days at two different storage temperatures (4°C and 25°C). In order to evaluate the versatility of these systems in in vivo applications, further stability studies in different biological fluids, such as human and bovine serum, artificial cerebrospinal fluid (aCSF), artificial saliva, simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), were conducted. This work has shown that Neem oil and Tween surfactants are able to form stable nanoemulsions suitable as nanodelivery systems for different administration routes.
New drug delivery systems: nanoemulsions and their potential applications / Hanieh, PATRIZIA NADIA; Pianella, Monica; Rinaldi, Federica; Carradori, Simone; Longhi, Catia; Mattia, Elena; Marianecci, Carlotta; Carafa, Maria. - (2016), pp. 84-84. (Intervento presentato al convegno Nanomedicine Viterbo 2016 tenutosi a Viterbo, Italy nel 21-23 Settembre 2016).
New drug delivery systems: nanoemulsions and their potential applications
HANIEH, PATRIZIA NADIA;PIANELLA, MONICA;RINALDI, FEDERICA;CARRADORI, Simone;LONGHI, Catia;MATTIA, Elena;MARIANECCI, CARLOTTA;CARAFA, Maria
2016
Abstract
Advanced drug delivery systems have been developed to overcome the major drawbacks associated with the conventional ones. The aim of our work is to develop nanoemulsions suitable for delivering bioactive substances. Nanoemulsions (NEs) are nano-sized emulsions, manufactured for improving the delivery of active pharmaceutical ingredients. These carriers are composed by oil and surfactants; in particular, natural oils can exhibit anti-inflammatory, antioxidant, antibacterial, antiviral, anticancer and/or tissue regenerative activity, due to the presence of polyphenols and tocopherols. The droplet size of NEs falls typically in the range 10–200 nm. They are kinetically more stable than microemulsions with no apparent flocculation or coalescence; they also improve intracellular penetration in biological tissues, bioavailability, tolerability and solubility of lipophilic drugs. As a result of the slow release of active compounds, the irritability described in tissues is low. NEs are also non-toxic and can be formulated for different applications, such as topical, ocular, intravenous, intranasal and oral delivery. In this study, nanoemulsions from Neem seed oil and Tween 20 or Tween 80 as non-ionic surfactants were prepared. In particular, Neem oil was selected since it shows many bioactive properties, such as antibacterial, antimalarial and antifungal activity. A mean droplet size ranging from 10–100 nm was obtained by modulating the oil/surfactant ratio. Several nanoemulsion formulations were characterized in terms of size and ζ-potential and physicochemical properties, such as microviscosity, polarity and turbidity, were evaluated. Furthermore stability studies were carried out for a period of 60 days at two different storage temperatures (4°C and 25°C). In order to evaluate the versatility of these systems in in vivo applications, further stability studies in different biological fluids, such as human and bovine serum, artificial cerebrospinal fluid (aCSF), artificial saliva, simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), were conducted. This work has shown that Neem oil and Tween surfactants are able to form stable nanoemulsions suitable as nanodelivery systems for different administration routes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
