Chitosan has been considered a good candidate for drug absorption enhancement in mucosal drug delivery (such as nasal, buccal, oral and gastrointestinal drug delivery system). Chitosan is a natural polysaccharide obtained by chitin deacetylation found in the exoskeleton of crustaceans. Chitosan mucoadhesive properties are due to the interaction between its protonated amino groups with the sialic acid residues of the mucus layers. Mucoadhesion is responsible of the enhancement in contact and residence time between mucosal membranes and drug carriers at the absorption sites. Furthermore, chitosan is also able to open reversibly tight junctions increasing the permeability via receptor-mediated signalling, enhancing the trans-epithelial transport through intercellular pathway. An interesting therapeutic strategy could be a combination between chitosan mucoahdesion and nanovesicles characteristics. In particular, niosomes are non-ionic surfactant vesicles capable to entrap both hydrophilic and lipophilic drugs, minimizing drug degradation and inactivation after administration, and increasing drug bioavailability and targeting to the pathological area. The aim of this work is the complexation between negatively charged niosomal vesicles and chitosan, a cationic mucoadhesive polysaccharide. In this study, three different formulation of unilamellar niosomal vesicles composed by a mixture of Tween 20 or Tween 21 or Span 20 with cholesterol and dicetyl phosphate have been prepared and characterized. The study of the aggregation behavior between the charged vesicles and chitosan has been carried out in terms of size and ζ-potential evaluation of the obtained hybrid structures and size and ζ-potential time stability of the selected samples. These experiments have been performed by dynamic light scattering (DLS) and Laser Doppler Electrophoresis measurements. The effect of chitosan charge ratio is investigated and discussed within the framework of the re-entrant condensation and charge inversion behavior.
CHITOSAN-COATED NON-IONIC SURFACTANT VESICLES: PREPARATION AND CHARACTERIZATION / Hanieh, PATRIZIA NADIA; Pianella, Monica; Rinaldi, Federica; Di Francesco, Martina; Marianecci, Carlotta; Carafa, Maria. - CD-ROM. - (2016), pp. 43-43. (Intervento presentato al convegno 10th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology tenutosi a Glasgow, United Kindom nel 4-7 Aprile 2016).
CHITOSAN-COATED NON-IONIC SURFACTANT VESICLES: PREPARATION AND CHARACTERIZATION
HANIEH, PATRIZIA NADIA;PIANELLA, MONICA;RINALDI, FEDERICA;MARIANECCI, CARLOTTA;CARAFA, Maria
2016
Abstract
Chitosan has been considered a good candidate for drug absorption enhancement in mucosal drug delivery (such as nasal, buccal, oral and gastrointestinal drug delivery system). Chitosan is a natural polysaccharide obtained by chitin deacetylation found in the exoskeleton of crustaceans. Chitosan mucoadhesive properties are due to the interaction between its protonated amino groups with the sialic acid residues of the mucus layers. Mucoadhesion is responsible of the enhancement in contact and residence time between mucosal membranes and drug carriers at the absorption sites. Furthermore, chitosan is also able to open reversibly tight junctions increasing the permeability via receptor-mediated signalling, enhancing the trans-epithelial transport through intercellular pathway. An interesting therapeutic strategy could be a combination between chitosan mucoahdesion and nanovesicles characteristics. In particular, niosomes are non-ionic surfactant vesicles capable to entrap both hydrophilic and lipophilic drugs, minimizing drug degradation and inactivation after administration, and increasing drug bioavailability and targeting to the pathological area. The aim of this work is the complexation between negatively charged niosomal vesicles and chitosan, a cationic mucoadhesive polysaccharide. In this study, three different formulation of unilamellar niosomal vesicles composed by a mixture of Tween 20 or Tween 21 or Span 20 with cholesterol and dicetyl phosphate have been prepared and characterized. The study of the aggregation behavior between the charged vesicles and chitosan has been carried out in terms of size and ζ-potential evaluation of the obtained hybrid structures and size and ζ-potential time stability of the selected samples. These experiments have been performed by dynamic light scattering (DLS) and Laser Doppler Electrophoresis measurements. The effect of chitosan charge ratio is investigated and discussed within the framework of the re-entrant condensation and charge inversion behavior.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
