Recent studies have suggested a possible correlation between obesity and adenovirus 36 (Adv36) infection in humans. Since information on adenoviral DNA presence in human adipose tissue are limited, we evaluated the presence of Adv36 DNA in adipose tissue of 21 adult overweight or obese patients. Total DNA was extracted from adipose tissue biopsies. Virus detection was performed using PCR protocols with primers against specific Adv36 fiber protein and the viral oncogenic E4-ORF1 protein nucleotide sequences. Sequences were aligned with the NCBI database and phylogenetic analyses were carried out with MEGA6 software. Adv36 DNA was found in 4 samples (19%). This study indicates that some individuals carry Adv36 in the visceral adipose tissue. Further studies are needed to determine the specific effect of Adv36 infection on adipocytes, the prevalence of Adv36 infection and its relationship with obesity in the perspective of developing a vaccine that could potentially prevent or mitigate infection.

Adenovirus 36 DNA in human adipose tissue / Ponterio, E; Cangemi, Roberto; Mariani, S; Casella, Giovanni; DE CESARE, Alessandro; Trovato, F. M; Garozzo, A; Gnessi, Lucio. - In: INTERNATIONAL JOURNAL OF OBESITY. - ISSN 1476-5497. - STAMPA. - 39:12(2015), pp. 1761-1764. [10.1038/ijo.2015.163]

Adenovirus 36 DNA in human adipose tissue

CANGEMI, ROBERTO;Mariani, S;CASELLA, GIOVANNI;DE CESARE, Alessandro;GNESSI, Lucio
2015

Abstract

Recent studies have suggested a possible correlation between obesity and adenovirus 36 (Adv36) infection in humans. Since information on adenoviral DNA presence in human adipose tissue are limited, we evaluated the presence of Adv36 DNA in adipose tissue of 21 adult overweight or obese patients. Total DNA was extracted from adipose tissue biopsies. Virus detection was performed using PCR protocols with primers against specific Adv36 fiber protein and the viral oncogenic E4-ORF1 protein nucleotide sequences. Sequences were aligned with the NCBI database and phylogenetic analyses were carried out with MEGA6 software. Adv36 DNA was found in 4 samples (19%). This study indicates that some individuals carry Adv36 in the visceral adipose tissue. Further studies are needed to determine the specific effect of Adv36 infection on adipocytes, the prevalence of Adv36 infection and its relationship with obesity in the perspective of developing a vaccine that could potentially prevent or mitigate infection.
2015
Obesity; adipose tissue; Adenovirus 36; PCR
01 Pubblicazione su rivista::01i Case report
Adenovirus 36 DNA in human adipose tissue / Ponterio, E; Cangemi, Roberto; Mariani, S; Casella, Giovanni; DE CESARE, Alessandro; Trovato, F. M; Garozzo, A; Gnessi, Lucio. - In: INTERNATIONAL JOURNAL OF OBESITY. - ISSN 1476-5497. - STAMPA. - 39:12(2015), pp. 1761-1764. [10.1038/ijo.2015.163]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/832761
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