A programmed packaging strategy to develop a multicomponent envelope-type nanoparticle system (MENS) is presented. To this end, we took specific advantage of using in-house tailored liposomes that have been recently shown to exhibit intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes and to enter the nucleus with extremely high efficiency. Transfection efficiency experiments on NIH 3T3 mouse fibroblasts indicate that MENS is a promising transfection candidate. (C) 2010 American Institute of Physics. [doi:10.1063/1.3427354]
Programmed packaging of multicomponent envelope-type nanoparticle system for gene delivery / Pozzi, Daniela; Marianecci, Carlotta; Carafa, Maria; Cristina, Marchini; Maura, Montani; Augusto, Amici; Caracciolo, Giulio. - In: APPLIED PHYSICS LETTERS. - ISSN 0003-6951. - 96:18(2010), pp. 183702-1-183702-3. [10.1063/1.3427354]
Programmed packaging of multicomponent envelope-type nanoparticle system for gene delivery
POZZI, DANIELA;MARIANECCI, CARLOTTA;CARAFA, Maria;CARACCIOLO, Giulio
2010
Abstract
A programmed packaging strategy to develop a multicomponent envelope-type nanoparticle system (MENS) is presented. To this end, we took specific advantage of using in-house tailored liposomes that have been recently shown to exhibit intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes and to enter the nucleus with extremely high efficiency. Transfection efficiency experiments on NIH 3T3 mouse fibroblasts indicate that MENS is a promising transfection candidate. (C) 2010 American Institute of Physics. [doi:10.1063/1.3427354]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
