Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.
Biologic characterization of pleural metastases from lung adenocarcinoma: description of the new DV90 cell line / Monti, F; Szymczuk, S; Chessa, L; Prudente, S; Nicoletti, G; Fattori, P P; Pini, E; Desiderio, F; Pasquini, E; Tison, V. - In: TUMORI. - ISSN 0300-8916. - 80:5(1994). [10.1177/030089169408000513]
Biologic characterization of pleural metastases from lung adenocarcinoma: description of the new DV90 cell line
Chessa, L;Prudente, S;
1994
Abstract
Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
