Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical ionization ion sources. Analysis and relative quantification of the non-polar volatile fraction were conducted by gas chromatography (GC) coupled to MS. FT-ICR MS confirmed significant differences in the polar metabolite composition, while GC-MS analyses confirmed slight fluctuations in the relative amount of major terpenes and terpenoids, known to play a key role in antimicrobial mechanisms. Oil in eater (O/W) nanoemulsions (NEs) composed by SEOs and Tween 20 or Tween 80 were prepared and analyzed in terms of hydrodynamic diameter, ζ-potential and polydispersity index. The results confirm the formation of stable NEs homogeneous in size. Minimum inhibitory and minimum bactericidal concentrations of SEOs were determined towards Gram-positive (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus haemolyticus) and Gram-negative clinical isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens). Commercial SEO showed strongest antibacterial activity, while SEO 3 was found to be the most active among the lab made extractions. MIC and MBC values ranged from 0.39 to 6.25 mg·mL-1. Furthermore, a SEO structured in NEs formulation was able to preserve and improve antimicrobial activity.

Satureja montana L. essential oils. Chemical profiles/phytochemical screening, antimicrobial activity and o/w nanoemulsion formulations / Maccelli, Alessandro; Vitanza, Luca; Imbriano, Anna; Fraschetti, Caterina; Filippi, Antonello; Goldoni, Paola; Maurizi, Linda; Ammendolia, Maria Grazia; Crestoni, Maria Elisa; Fornarini, Simonetta; Menghini, Luigi; Carafa, Maria; Marianecci, Carlotta; Longhi, Catia; Rinaldi, Federica. - In: PHARMACEUTICS. - ISSN 1999-4923. - 12:1(2020). [10.3390/pharmaceutics12010007]

Satureja montana L. essential oils. Chemical profiles/phytochemical screening, antimicrobial activity and o/w nanoemulsion formulations

Maccelli, Alessandro;Vitanza, Luca;Fraschetti, Caterina;Filippi, Antonello;Goldoni, Paola;Maurizi, Linda;Crestoni, Maria Elisa;Fornarini, Simonetta;Carafa, Maria;Marianecci, Carlotta;Longhi, Catia
;
Rinaldi, Federica
2020

Abstract

Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical ionization ion sources. Analysis and relative quantification of the non-polar volatile fraction were conducted by gas chromatography (GC) coupled to MS. FT-ICR MS confirmed significant differences in the polar metabolite composition, while GC-MS analyses confirmed slight fluctuations in the relative amount of major terpenes and terpenoids, known to play a key role in antimicrobial mechanisms. Oil in eater (O/W) nanoemulsions (NEs) composed by SEOs and Tween 20 or Tween 80 were prepared and analyzed in terms of hydrodynamic diameter, ζ-potential and polydispersity index. The results confirm the formation of stable NEs homogeneous in size. Minimum inhibitory and minimum bactericidal concentrations of SEOs were determined towards Gram-positive (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus haemolyticus) and Gram-negative clinical isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens). Commercial SEO showed strongest antibacterial activity, while SEO 3 was found to be the most active among the lab made extractions. MIC and MBC values ranged from 0.39 to 6.25 mg·mL-1. Furthermore, a SEO structured in NEs formulation was able to preserve and improve antimicrobial activity.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/1346683
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