In vitro lymphocytes from healthy donors with crude (HCMV), heat inactivated (HCMVi) and infected human fetal fibroblasts (HCMVFF) were stimulated. In all the experiments peak titers of alfa Interferon (IFN) were present 24 h after exposure to antigens. On the other hand, at least after seven days, only lymphocytes of seropositive donors stimulated by HCMVFF and HCMV showed gamma IFN production. Our HCMVFF gave much higher IFN yields than free virus. Our data suggest that HCMVFF and HCMV are recognised by lymphocytes of HCMV specific memory and HCMVFF give additional and more reproducible information on HCMV specific cellular immunity.
In vitro interferon production: induction in human leukocytes after exposure to cytomegalovirus antigens / Lun, Maria Teresa; Lorino, Giulia; Gaeta, Aurelia; Dessy, P; Cipriani, Paola; Filadoro, Pasquale; Capobianchi, Maria Rosaria; DI MARCO, Paola; Mancini, Carlo. - In: MICROBIOLOGICA. - ISSN 0391-5352. - 9 (4):(1986), pp. 399-404.
In vitro interferon production: induction in human leukocytes after exposure to cytomegalovirus antigens
LUN, Maria Teresa;LORINO, Giulia;GAETA, Aurelia;CIPRIANI, Paola;FILADORO, Pasquale;CAPOBIANCHI, Maria Rosaria;DI MARCO, Paola;MANCINI, Carlo
1986
Abstract
In vitro lymphocytes from healthy donors with crude (HCMV), heat inactivated (HCMVi) and infected human fetal fibroblasts (HCMVFF) were stimulated. In all the experiments peak titers of alfa Interferon (IFN) were present 24 h after exposure to antigens. On the other hand, at least after seven days, only lymphocytes of seropositive donors stimulated by HCMVFF and HCMV showed gamma IFN production. Our HCMVFF gave much higher IFN yields than free virus. Our data suggest that HCMVFF and HCMV are recognised by lymphocytes of HCMV specific memory and HCMVFF give additional and more reproducible information on HCMV specific cellular immunity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
