Main purpose of the work is assembling, testing and optimizing new disposable amperometric biosensors to analyze substances in different application fields as agribusiness, clinical chemistry and environment protection. Many kinds of modified electrodes have been prepared and tested to build portable devices to analyze quickly many analytes, in a simple and cost-effective manner. Bare electrodes of the screen-printed type, with silver as reference, have been used for modification. The glassy carbon electrodes with multi-walled carbon nanotubes or graphene or gold nanoparticles depositions were modified with generation IV ionic liquids. Choline as cation and amino acids, such as glycine, serine, phenylalanine and histidine, as anions have been employed for these ionic liquids. The presence of nanostructured materials on the electrode brings an increased contact surface between analytes and receptor and, consequently, an amplification of the amperometric signal and a better sensibility. Moreover the use of new ionic liquids of generation IV, biologically friendly and water soluble, improves the electronic transfer, facilitating and strengthening the redox reaction nearby the electrode. By immobilizing the proper enzymes onto the modified electrode surface, different compounds of analytical interest can be determined by means of sensitive, properly designed amperometric biosensors. Analytes such as antioxidant components in extra-virgin olive oils, alcohols in beverages and glucose in food matrices have been tested, using a suitable enzyme: microbial lipase, alcohol dehydrogenase and glucose oxidase, respectively.

Biologically friendly room temperature ionic liquids and nanomaterials for the development of innovative enzymatic biosensors / Zappi, Daniele; Caminiti, Ruggero; Ingo, G. M.; Sadun, C.; Tortolini, C.; Antonelli, Marta Letizia. - In: TALANTA. - ISSN 0039-9140. - STAMPA. - 175:(2017), pp. 566-572. [10.1016/j.talanta.2017.07.081]

Biologically friendly room temperature ionic liquids and nanomaterials for the development of innovative enzymatic biosensors

ZAPPI, DANIELE
;
Caminiti, Ruggero;Sadun, C.;Tortolini, C.;Antonelli, Marta Letizia
2017

Abstract

Main purpose of the work is assembling, testing and optimizing new disposable amperometric biosensors to analyze substances in different application fields as agribusiness, clinical chemistry and environment protection. Many kinds of modified electrodes have been prepared and tested to build portable devices to analyze quickly many analytes, in a simple and cost-effective manner. Bare electrodes of the screen-printed type, with silver as reference, have been used for modification. The glassy carbon electrodes with multi-walled carbon nanotubes or graphene or gold nanoparticles depositions were modified with generation IV ionic liquids. Choline as cation and amino acids, such as glycine, serine, phenylalanine and histidine, as anions have been employed for these ionic liquids. The presence of nanostructured materials on the electrode brings an increased contact surface between analytes and receptor and, consequently, an amplification of the amperometric signal and a better sensibility. Moreover the use of new ionic liquids of generation IV, biologically friendly and water soluble, improves the electronic transfer, facilitating and strengthening the redox reaction nearby the electrode. By immobilizing the proper enzymes onto the modified electrode surface, different compounds of analytical interest can be determined by means of sensitive, properly designed amperometric biosensors. Analytes such as antioxidant components in extra-virgin olive oils, alcohols in beverages and glucose in food matrices have been tested, using a suitable enzyme: microbial lipase, alcohol dehydrogenase and glucose oxidase, respectively.
alcohol dehydrogenase; enzymatic biosensor; glucose oxidase; ionic liquid; lipase; nanostructured electrode; chemistry (all)
01 Pubblicazione su rivista::01a Articolo in rivista
Biologically friendly room temperature ionic liquids and nanomaterials for the development of innovative enzymatic biosensors / Zappi, Daniele; Caminiti, Ruggero; Ingo, G. M.; Sadun, C.; Tortolini, C.; Antonelli, Marta Letizia. - In: TALANTA. - ISSN 0039-9140. - STAMPA. - 175:(2017), pp. 566-572. [10.1016/j.talanta.2017.07.081]
File allegati a questo prodotto
File Dimensione Formato  
Zappi_Biologically-friendly_2017.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.2 MB
Formato Adobe PDF
1.2 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1015035
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 27
  • ???jsp.display-item.citation.isi??? 25
social impact