Background: Whether intestinal microbiota and metabolic profiling may be altered in patients with SUDD is unknown. Patients and Methods: Stool samples from 44 consecutive women [15 patients with SUDD, 13 with asymptomatic diverticulosis (AD), and 16 healthy controls (HCs)] were analyzed. Real-time polymerase chain reaction was used to quantify targeted microorganisms. High-resolution proton nuclear magnetic resonance spectroscopy associated with multivariate analysis with partial least-square discriminant analysis (PLS-DA) was applied on the metabolite data set. Results: The overall bacterial quantity did not differ among the 3 groups (P=0.449), with no difference in Bacteroides/Prevotella, Clostridium coccoides, Bifidobacterium, Lactobacillus, and Escherichia coli subgroups. The amount of Akkermansia muciniphila species was significantly different between HC, AD, and SUDD subjects (P=0.017). PLS-DA analysis of nuclear magnetic resonance -based metabolomics associated with microbiological data showed significant discrimination between HCs and AD patients (R2=0.733; Q2=0.383; P<0.05, LV=2). PLS analysis showed lower N-acetyl compound and isovalerate levels in AD, associated with higher levels of A. municiphila, as compared with the HC group. PLS-DA applied on AD and SUDD samples showed a good discrimination between these 2 groups (R2=0.69; Q2=0.35; LV=2). SUDD patients were characterized by low levels of valerate, butyrate, and choline and by high levels of N-acetyl derivatives and U1. Conclusions: SUDD and AD do not show colonic bacterial overgrowth, but a significant difference in the levels of fecal A. muciniphila was observed. Moreover, increasing expression of some metabolites as expression of different AD and SUDD metabolic activity was found.

Assessment of fecal microbiota and fecal metabolome in symptomatic uncomplicated diverticular disease of the colon / Tursi, Antonio; Mastromarino, Paola; Capobianco, Daniela; Elisei, Walter; Miccheli, Alfredo; Capuani, Giorgio; Tomassini, Alberta; Campagna, Giuseppe; Picchio, Marcello; Giorgetti, Gian Marco; Fabiocchi, Federica; Brandimarte, Giovanni. - In: JOURNAL OF CLINICAL GASTROENTEROLOGY. - ISSN 0192-0790. - STAMPA. - 50:Supp 1(2016), pp. S9-S12. [10.1097/MCG.0000000000000626]

Assessment of fecal microbiota and fecal metabolome in symptomatic uncomplicated diverticular disease of the colon

MASTROMARINO, Paola;CAPOBIANCO, DANIELA;MICCHELI, Alfredo;CAPUANI, Giorgio;TOMASSINI, Alberta;CAMPAGNA, GIUSEPPE;
2016

Abstract

Background: Whether intestinal microbiota and metabolic profiling may be altered in patients with SUDD is unknown. Patients and Methods: Stool samples from 44 consecutive women [15 patients with SUDD, 13 with asymptomatic diverticulosis (AD), and 16 healthy controls (HCs)] were analyzed. Real-time polymerase chain reaction was used to quantify targeted microorganisms. High-resolution proton nuclear magnetic resonance spectroscopy associated with multivariate analysis with partial least-square discriminant analysis (PLS-DA) was applied on the metabolite data set. Results: The overall bacterial quantity did not differ among the 3 groups (P=0.449), with no difference in Bacteroides/Prevotella, Clostridium coccoides, Bifidobacterium, Lactobacillus, and Escherichia coli subgroups. The amount of Akkermansia muciniphila species was significantly different between HC, AD, and SUDD subjects (P=0.017). PLS-DA analysis of nuclear magnetic resonance -based metabolomics associated with microbiological data showed significant discrimination between HCs and AD patients (R2=0.733; Q2=0.383; P<0.05, LV=2). PLS analysis showed lower N-acetyl compound and isovalerate levels in AD, associated with higher levels of A. municiphila, as compared with the HC group. PLS-DA applied on AD and SUDD samples showed a good discrimination between these 2 groups (R2=0.69; Q2=0.35; LV=2). SUDD patients were characterized by low levels of valerate, butyrate, and choline and by high levels of N-acetyl derivatives and U1. Conclusions: SUDD and AD do not show colonic bacterial overgrowth, but a significant difference in the levels of fecal A. muciniphila was observed. Moreover, increasing expression of some metabolites as expression of different AD and SUDD metabolic activity was found.
Diverticular disease; Fecal metabolome; Fecal microbiota; Gastroenterology
01 Pubblicazione su rivista::01a Articolo in rivista
Assessment of fecal microbiota and fecal metabolome in symptomatic uncomplicated diverticular disease of the colon / Tursi, Antonio; Mastromarino, Paola; Capobianco, Daniela; Elisei, Walter; Miccheli, Alfredo; Capuani, Giorgio; Tomassini, Alberta; Campagna, Giuseppe; Picchio, Marcello; Giorgetti, Gian Marco; Fabiocchi, Federica; Brandimarte, Giovanni. - In: JOURNAL OF CLINICAL GASTROENTEROLOGY. - ISSN 0192-0790. - STAMPA. - 50:Supp 1(2016), pp. S9-S12. [10.1097/MCG.0000000000000626]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/953415
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