The first thermophilic eubacterial glutamate dehydrogenase was purified to homogeneity from Bacillus acidocaldarius to compare its molecular properties with those of the glutamate dehydrogenase from thermophilic archaea. Glutamate dehydrogenase represents 2% of the total soluble proteins of B. acidocaldarius, an amount that may suggest an important role for this enzyme in nitrogen metabolism of the thermophilic eubacterium. The protein is a hexamer (subunit mass 48 kDa) and undergoes dissociation during gel filtration analysis. Isoelectric focusing of the purified enzyme indicated a pI of 4.5. The enzyme is strictly specific for NAD, 2-oxoglutarate, and L-glutamate. The thermal stability of B. acidocaldarius glutamate dehydrogenase is dependent on protein concentration.
NAD-DEPENDENT GLUTAMATE DEHYDROGENASE FROM THE THERMOPHYLIC EUBACTERIUM Bacillus acidocaldarius / Consalvi, Valerio; Chiaraluce, Roberta; S., Millevoi; A., Pasquo; Politi, Laura; M., DE ROSA; Scandurra, Roberto. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY. - ISSN 1096-4959. - 109:(1994), pp. 691-699. [10.1016/0305-0491(94)90132-5]
NAD-DEPENDENT GLUTAMATE DEHYDROGENASE FROM THE THERMOPHYLIC EUBACTERIUM Bacillus acidocaldarius.
CONSALVI, Valerio;CHIARALUCE, Roberta;POLITI, Laura;SCANDURRA, Roberto
1994
Abstract
The first thermophilic eubacterial glutamate dehydrogenase was purified to homogeneity from Bacillus acidocaldarius to compare its molecular properties with those of the glutamate dehydrogenase from thermophilic archaea. Glutamate dehydrogenase represents 2% of the total soluble proteins of B. acidocaldarius, an amount that may suggest an important role for this enzyme in nitrogen metabolism of the thermophilic eubacterium. The protein is a hexamer (subunit mass 48 kDa) and undergoes dissociation during gel filtration analysis. Isoelectric focusing of the purified enzyme indicated a pI of 4.5. The enzyme is strictly specific for NAD, 2-oxoglutarate, and L-glutamate. The thermal stability of B. acidocaldarius glutamate dehydrogenase is dependent on protein concentration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
