Human papillomavirus (HPV) is estimated to be the cause of 40-80% of the squamous cell carcinoma of the oropharynx but only of a small fraction of the oral cavity cancers. The prevalence of oral HPV infection has significantly increased in the last decade, raising concerns about the HPV role in progression of oral potentially malignant disorders (OPMD) toward squamous cell carcinomas. We sought to study HPV infection in patients with oral lesions, and in control individuals, using non-invasive and site-specific oral brushing and sensitive molecular methods. HPV DNA positivity and viral loads were evaluated in relation to patient data and clinical diagnosis. We enrolled 116 individuals attending Dental Clinics: 62 patients with benign oral lesions (e.g. fibromas, papillomatosis, ulcers) or OPMD (e.g. lichen, leukoplakia) and 54 controls. Oral cells were collected with Cytobrush and HPV-DNA detected with quantitative real-time PCR (qPCR) for the more common high-risk (HR) and low-risk (LR) genotypes. HPV detection rate, percentage of HR HPVs and HPV-DNA loads (namely HPV16 and in particular, HPV18) were significantly higher in patients than in controls. Lichen planus cases had the highest HPV positive rate (75.0%), hairy leukoplakia the lowest (33.3%). This study detected unexpectedly high rates of HPV infection in cells of the oral mucosa. The elevated HR HPV loads found in OPMD suggest the effectiveness of qPCR in testing oral lesions. Prospective studies are needed to establish whether elevated viral loads represent a clinically useful marker of the risk of malignant progression.

Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders / PIERANGELI, Alessandra; CANNELLA, FABIANA; SCAGNOLARI, CAROLINA; GENTILE, Massimo; SCIANDRA, ILARIA; ANTONELLI, Guido; Ciolfi, Chiara; RUSSO, CLAUDIA; PALAIA, GASPARE; ROMEO, Umberto; POLIMENI, Antonella. - In: CLINICAL MICROBIOLOGY AND INFECTION. - ISSN 1198-743X. - STAMPA. - 22:1(2016), pp. 95.e9-95.e15. [10.1016/j.cmi.2015.09.011]

Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders

PIERANGELI, Alessandra
Primo
;
CANNELLA, FABIANA
Secondo
;
SCAGNOLARI, CAROLINA;GENTILE, Massimo;SCIANDRA, ILARIA;ANTONELLI, Guido
;
Ciolfi, Chiara;RUSSO, CLAUDIA;PALAIA, GASPARE;ROMEO, Umberto
Penultimo
;
POLIMENI, Antonella
Ultimo
2016

Abstract

Human papillomavirus (HPV) is estimated to be the cause of 40-80% of the squamous cell carcinoma of the oropharynx but only of a small fraction of the oral cavity cancers. The prevalence of oral HPV infection has significantly increased in the last decade, raising concerns about the HPV role in progression of oral potentially malignant disorders (OPMD) toward squamous cell carcinomas. We sought to study HPV infection in patients with oral lesions, and in control individuals, using non-invasive and site-specific oral brushing and sensitive molecular methods. HPV DNA positivity and viral loads were evaluated in relation to patient data and clinical diagnosis. We enrolled 116 individuals attending Dental Clinics: 62 patients with benign oral lesions (e.g. fibromas, papillomatosis, ulcers) or OPMD (e.g. lichen, leukoplakia) and 54 controls. Oral cells were collected with Cytobrush and HPV-DNA detected with quantitative real-time PCR (qPCR) for the more common high-risk (HR) and low-risk (LR) genotypes. HPV detection rate, percentage of HR HPVs and HPV-DNA loads (namely HPV16 and in particular, HPV18) were significantly higher in patients than in controls. Lichen planus cases had the highest HPV positive rate (75.0%), hairy leukoplakia the lowest (33.3%). This study detected unexpectedly high rates of HPV infection in cells of the oral mucosa. The elevated HR HPV loads found in OPMD suggest the effectiveness of qPCR in testing oral lesions. Prospective studies are needed to establish whether elevated viral loads represent a clinically useful marker of the risk of malignant progression.
2016
human papillomavirus; leukoplakia; lichen planus; oral lesions; oral potentially malignant disorders; papillomatosis; squamous cell carcinomas of the oral cavity
01 Pubblicazione su rivista::01a Articolo in rivista
Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders / PIERANGELI, Alessandra; CANNELLA, FABIANA; SCAGNOLARI, CAROLINA; GENTILE, Massimo; SCIANDRA, ILARIA; ANTONELLI, Guido; Ciolfi, Chiara; RUSSO, CLAUDIA; PALAIA, GASPARE; ROMEO, Umberto; POLIMENI, Antonella. - In: CLINICAL MICROBIOLOGY AND INFECTION. - ISSN 1198-743X. - STAMPA. - 22:1(2016), pp. 95.e9-95.e15. [10.1016/j.cmi.2015.09.011]
File allegati a questo prodotto
File Dimensione Formato  
Pierangeli_Frequent_2016.pdf

accesso aperto

Note: https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(15)00865-4/fulltext
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 261.63 kB
Formato Adobe PDF
261.63 kB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/835216
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 10
social impact