A new composite broth medium combining o-nitrophenyl-i-D-galactopyranoside (ONPG) and urease and indole tests in a single tube is described. High-level agreement with individual conventional tests was recorded in comparative studies with 2,412 cultures of members of the family Enterobacteriaceae, i.e., 100% agreement with the exception of Hafnia spp. (96.3% agreement) for the ONPG test and Citrobacter, Enterobacter, and Hafnia spp. (75, 86.4, and 98.2% agreement, respectively) for the urease test. The new medium seems especially promising as a screen for Salmonella subgroup I which encompasses most pathogenic Salmonella species other than the Arizona subgroup.
ORTHO-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE UREASE INDOLE BROTH, A NEW COMPOSITE TUBE MEDIUM FOR SALMONELLA SCREENING / Berlutti, Francesca; Dainelli, B; Martini, A; Thaller, Mc. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - STAMPA. - 24:(1986), pp. 650-651.
ORTHO-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE UREASE INDOLE BROTH, A NEW COMPOSITE TUBE MEDIUM FOR SALMONELLA SCREENING
BERLUTTI, Francesca;
1986
Abstract
A new composite broth medium combining o-nitrophenyl-i-D-galactopyranoside (ONPG) and urease and indole tests in a single tube is described. High-level agreement with individual conventional tests was recorded in comparative studies with 2,412 cultures of members of the family Enterobacteriaceae, i.e., 100% agreement with the exception of Hafnia spp. (96.3% agreement) for the ONPG test and Citrobacter, Enterobacter, and Hafnia spp. (75, 86.4, and 98.2% agreement, respectively) for the urease test. The new medium seems especially promising as a screen for Salmonella subgroup I which encompasses most pathogenic Salmonella species other than the Arizona subgroup.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
