The usefulness of β-GUI (ONPG-urease-indolemedium), coupled with triple sugar iron agar (TSI), Kligler iron agar (KIA) and lysine iron agar (LIA), respectively, for the early detection of Salmonella, Shigella and Yersinia was evaluated and compared with the LIA combined with both TSI and KIA. The study was performed on 749 selected, lactose-negative Enterobacteriaceae isolates. The most effective combination was LIA-β-GUI, which showed the highest efficiency for Shigella Yersinia presumptive recognition. It also yielded good results for Salmonella, leading to the further examination of only four cultures more than TSI-β-GUI which was the best for Salmonellascreening. Moreover, out of the 749 lactose-negative isolates, it considered as a ‘suspicious entericpathogen’ the lowest number of strains (452, 60.3%). Out of these, 416 (55.5% of the total strains and 92.0% of those yet to be tested) actually belonged to Salmonella, Shigella or Yersinia genus.
O-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE-UREASE-INDOLE MEDIUM IN THE SCREENING OF ENTERIC PATHOGENS / M. C., Thaller; Berlutti, Francesca; R., Pezzi. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - STAMPA. - 8:(1988), pp. 227-234. [10.1016/0167-7012(88)90005-x]
O-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE-UREASE-INDOLE MEDIUM IN THE SCREENING OF ENTERIC PATHOGENS
BERLUTTI, Francesca;
1988
Abstract
The usefulness of β-GUI (ONPG-urease-indolemedium), coupled with triple sugar iron agar (TSI), Kligler iron agar (KIA) and lysine iron agar (LIA), respectively, for the early detection of Salmonella, Shigella and Yersinia was evaluated and compared with the LIA combined with both TSI and KIA. The study was performed on 749 selected, lactose-negative Enterobacteriaceae isolates. The most effective combination was LIA-β-GUI, which showed the highest efficiency for Shigella Yersinia presumptive recognition. It also yielded good results for Salmonella, leading to the further examination of only four cultures more than TSI-β-GUI which was the best for Salmonellascreening. Moreover, out of the 749 lactose-negative isolates, it considered as a ‘suspicious entericpathogen’ the lowest number of strains (452, 60.3%). Out of these, 416 (55.5% of the total strains and 92.0% of those yet to be tested) actually belonged to Salmonella, Shigella or Yersinia genus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
