In this paper, we describe a reduced sequence of identification that includes T-mod medium, a selective and differential isolation medium which allows accurate presumptive identification of the most common gramnegative bacteria encountered in urine samples. The present study, performed on bacteria isolated from 1,762 independent urine samples, has shown that a few selected tests (lysine and ornithine decarboxylase, urease and trehalose fermentation tests) improve the identification accuracy of T-mod, making it possible both to identify the less frequent species and to prevent some misidentifications of Klebsiella pneumoniae and Proteus mirabilis. The proposed work flow agreed with conventional identification protocols to a 99.3% extent and allowed identification of 87.4% of the isolates directly from the primary plate, 11.4% after 1 to 3 additional tests, and 1.2% after an identification gallery.
T-MOD PATHWAY, A REDUCED SEQUENCE FOR IDENTIFICATION OF GRAM-NEGATIVE URINARY-TRACT PATHOGENS / Berlutti, Francesca; Thaller, Mc; Dainelli, B; Pezzi, R.. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - STAMPA. - 27:(1989), pp. 1646-1649.
T-MOD PATHWAY, A REDUCED SEQUENCE FOR IDENTIFICATION OF GRAM-NEGATIVE URINARY-TRACT PATHOGENS
BERLUTTI, Francesca;
1989
Abstract
In this paper, we describe a reduced sequence of identification that includes T-mod medium, a selective and differential isolation medium which allows accurate presumptive identification of the most common gramnegative bacteria encountered in urine samples. The present study, performed on bacteria isolated from 1,762 independent urine samples, has shown that a few selected tests (lysine and ornithine decarboxylase, urease and trehalose fermentation tests) improve the identification accuracy of T-mod, making it possible both to identify the less frequent species and to prevent some misidentifications of Klebsiella pneumoniae and Proteus mirabilis. The proposed work flow agreed with conventional identification protocols to a 99.3% extent and allowed identification of 87.4% of the isolates directly from the primary plate, 11.4% after 1 to 3 additional tests, and 1.2% after an identification gallery.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.