Food safety is a crucial topic that is a major concern both to consumers and authorities worldwide. A particular concern is primarily directed to some sensitive group of consumers that include infants or people who are affected by disease. The aim of this study was to assess the risk of Aflatoxin M1 (AFM1) exposure by quantifying such mycotoxin in leading-brand infant formula milk products. Aflatoxins are toxic secondary metabolites produced by A. flavus and A. parasiticus fungal species. With regards to Aflatoxin M1 detection in milk and milk powder, the reference method UNI EN ISO 14501 was adopted with minor modifications. To this extent, the analytical method using immunoaffinity column clean-up and high-performance liquid chromatography (HPLC) with fluorimetric detection was preliminarily in-house validated, according to Eurachem guidelines. The obtained validation parameters (repeatability, reproducibility, accuracy) were compliant with the criteria as defined in the EU legislation (Regulation EC n. 401/2006).
Aflatoxin M1 detection in Italian infant formulas for infants and young children / Carlo, Brera; Colicchia, Sonia; Francesca, Debegnach; Emanuela, Gregori; Barbara De, Santis. - STAMPA. - (2014). (Intervento presentato al convegno The 8th World Mycotoxin Forum tenutosi a Vienna nel 10-12 Novembre 2014).
Aflatoxin M1 detection in Italian infant formulas for infants and young children
COLICCHIA, SONIA;
2014
Abstract
Food safety is a crucial topic that is a major concern both to consumers and authorities worldwide. A particular concern is primarily directed to some sensitive group of consumers that include infants or people who are affected by disease. The aim of this study was to assess the risk of Aflatoxin M1 (AFM1) exposure by quantifying such mycotoxin in leading-brand infant formula milk products. Aflatoxins are toxic secondary metabolites produced by A. flavus and A. parasiticus fungal species. With regards to Aflatoxin M1 detection in milk and milk powder, the reference method UNI EN ISO 14501 was adopted with minor modifications. To this extent, the analytical method using immunoaffinity column clean-up and high-performance liquid chromatography (HPLC) with fluorimetric detection was preliminarily in-house validated, according to Eurachem guidelines. The obtained validation parameters (repeatability, reproducibility, accuracy) were compliant with the criteria as defined in the EU legislation (Regulation EC n. 401/2006).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
