In this assay for oxalate in urine,oxalateis adsorbed from the urine onto graphitized carbon black (Carbopack B). After desorption and removal of the solvent, oxalic acid is gas-chromatographically measured after being derivatized with BF3/methanol. Chromatography is on Carbopack B/polyethylene glycol (M. 20 000), 93.7/6.3 by weight. The lower limit of detection of urinary oxalate is about 3 mg/L (CV _ 3.6%). A series of oxalate determinations in 24-h urine samples of 15 subjects gave a mean of 47.1 (SD 15.4) mg/24 h, with an analytical recovery of 97.4% (SD 3.0%, range 93.6-102.4%). Total analysis time for one sample is about 2 h.
SIMPLE, RELIABLE CHROMATOGRAPHIC MEASUREMENT OF OXALATE IN URINE / A., Di Corcia; Samperi, Roberto; Vinci, Giuliana; G., D'Ascenzo. - In: CLINICAL CHEMISTRY. - ISSN 1056-599X. - STAMPA. - 28:(1982), pp. 1457-1460.
SIMPLE, RELIABLE CHROMATOGRAPHIC MEASUREMENT OF OXALATE IN URINE
SAMPERI, Roberto;VINCI, Giuliana;
1982
Abstract
In this assay for oxalate in urine,oxalateis adsorbed from the urine onto graphitized carbon black (Carbopack B). After desorption and removal of the solvent, oxalic acid is gas-chromatographically measured after being derivatized with BF3/methanol. Chromatography is on Carbopack B/polyethylene glycol (M. 20 000), 93.7/6.3 by weight. The lower limit of detection of urinary oxalate is about 3 mg/L (CV _ 3.6%). A series of oxalate determinations in 24-h urine samples of 15 subjects gave a mean of 47.1 (SD 15.4) mg/24 h, with an analytical recovery of 97.4% (SD 3.0%, range 93.6-102.4%). Total analysis time for one sample is about 2 h.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
