A specific method for measuring concentrations of cortisol in serum, by preliminary isolation on a "minicolumn" followed by elution and determination by liquid chromatography, is described. This assay requires only 500μl of serum. The limit of determination of cortisol was found to be 5 μg l. The analytical recovery of cortisol added to serum ranged from 98 to 102%. The coefficients of variation ranged from 2.4 to 3.4% (within-day) and from 3.6 to 8.8% (day-to-day), depending on the cortisol concentration. The method compares well with a commonly used radioimmunological method.
Sample-pretreatment procedure for routine liquid chromatographic assay of serum cortisol / Lagana', Aldo; Rotatori, Mauro; Vinci, Giuliana; M. P., Colombini; Curini, Roberta. - In: TALANTA. - ISSN 0039-9140. - STAMPA. - 33:4(1986), pp. 325-328. [10.1016/0039-9140(86)80082-0]
Sample-pretreatment procedure for routine liquid chromatographic assay of serum cortisol
LAGANA', Aldo;ROTATORI, MAURO;VINCI, Giuliana;CURINI, Roberta
1986
Abstract
A specific method for measuring concentrations of cortisol in serum, by preliminary isolation on a "minicolumn" followed by elution and determination by liquid chromatography, is described. This assay requires only 500μl of serum. The limit of determination of cortisol was found to be 5 μg l. The analytical recovery of cortisol added to serum ranged from 98 to 102%. The coefficients of variation ranged from 2.4 to 3.4% (within-day) and from 3.6 to 8.8% (day-to-day), depending on the cortisol concentration. The method compares well with a commonly used radioimmunological method.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
