Background: NADPH-oxidase-2 up-regulation has been suggested in liver damage perpetuation via an oxidative stress-mediated mechanism. n-6/n-3 polyunsaturated fatty acids ratio derangement has been reported in liver disease. Aim: To explore polyunsaturated fatty acids balance and its interplay with platelet oxidative stress in liver cirrhosis. Methods: A cross-sectional study in 51 cirrhotic patients and sex- and age-matched controls was performed. Serum polyunsaturated fatty acids and oxidative stress markers (urinary isoprostanes and serum soluble NADPH-oxidase-2-derived peptide) were measured. The effect on platelet oxidative stress of n-6/n-3 polyunsaturated fatty acids ratio in vitro and in vivo (1-week supplementation with 3 g/daily n-3-polyunsaturated fatty acids) was tested. Results: Compared to controls, cirrhotic patients had significantly higher n-6/n-3 polyunsaturated fatty acids ratio. n-6/n-3 polyunsaturated fatty acids ratio correlated significantly with disease severity and oxidative stress markers. In vitro experiments showed that in Child-Pugh C patients' platelets incubation with low n-6/n-3 polyunsaturated fatty acids ratio resulted in dose-dependent decrease of radical oxigen species (-39%), isoprostanes (-25%) and NADPH-oxidase-2 regulation (-51%). n-3 polyunsaturated fatty acids supplemented patients showed significant oxidative stress indexes reduction. Conclusions: In cirrhosis, n-6/n-3 polyunsaturated fatty acids imbalance up-regulates platelet NADPH-oxidase-2 with ensuing oxidative stress. Further study to evaluate if n-3 supplementation may reduce disease progression is warranted. © 2014 Editrice Gastroenterologica Italiana S.r.l.
Polyunsaturated fatty acids balance affects platelet NOX2 activity in patients with liver cirrhosis / Basili, Stefania; Raparelli, Valeria; Napoleone, Laura; DEL BEN, Maria; Merli, Manuela; Riggio, Oliviero; Nocella, Cristina; Carnevale, Roberto; Pignatelli, Pasquale; Violi, Francesco; Angelico, Francesco; Amoroso, Daria; Bartimoccia, Simona; Vito, Lelio Burgio; Calabrese, CINZIA MYRIAM; Alessia, Carboni; Olivia, De Felice; DI SANTO, Serena; Giannelli, Valerio; Lucidi, Cristina; Nigro, Carmen; Pignataro, FRANCESCA SERENA; Proietti, Marco; Roberta, Russo; Talerico, Giovanni. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - STAMPA. - 46:7(2014), pp. 632-638. [10.1016/j.dld.2014.02.021]
Polyunsaturated fatty acids balance affects platelet NOX2 activity in patients with liver cirrhosis
BASILI, Stefania;RAPARELLI, Valeria;NAPOLEONE, LAURA;DEL BEN, Maria;MERLI, Manuela;RIGGIO, Oliviero;NOCELLA, CRISTINA;CARNEVALE, Roberto;PIGNATELLI, Pasquale;VIOLI, Francesco;ANGELICO, Francesco;AMOROSO, DARIA;BARTIMOCCIA, SIMONA;CALABRESE, CINZIA MYRIAM;DI SANTO, SERENA;GIANNELLI, VALERIO;LUCIDI, CRISTINA;NIGRO, CARMEN;PIGNATARO, FRANCESCA SERENA;PROIETTI, Marco;TALERICO, GIOVANNI
2014
Abstract
Background: NADPH-oxidase-2 up-regulation has been suggested in liver damage perpetuation via an oxidative stress-mediated mechanism. n-6/n-3 polyunsaturated fatty acids ratio derangement has been reported in liver disease. Aim: To explore polyunsaturated fatty acids balance and its interplay with platelet oxidative stress in liver cirrhosis. Methods: A cross-sectional study in 51 cirrhotic patients and sex- and age-matched controls was performed. Serum polyunsaturated fatty acids and oxidative stress markers (urinary isoprostanes and serum soluble NADPH-oxidase-2-derived peptide) were measured. The effect on platelet oxidative stress of n-6/n-3 polyunsaturated fatty acids ratio in vitro and in vivo (1-week supplementation with 3 g/daily n-3-polyunsaturated fatty acids) was tested. Results: Compared to controls, cirrhotic patients had significantly higher n-6/n-3 polyunsaturated fatty acids ratio. n-6/n-3 polyunsaturated fatty acids ratio correlated significantly with disease severity and oxidative stress markers. In vitro experiments showed that in Child-Pugh C patients' platelets incubation with low n-6/n-3 polyunsaturated fatty acids ratio resulted in dose-dependent decrease of radical oxigen species (-39%), isoprostanes (-25%) and NADPH-oxidase-2 regulation (-51%). n-3 polyunsaturated fatty acids supplemented patients showed significant oxidative stress indexes reduction. Conclusions: In cirrhosis, n-6/n-3 polyunsaturated fatty acids imbalance up-regulates platelet NADPH-oxidase-2 with ensuing oxidative stress. Further study to evaluate if n-3 supplementation may reduce disease progression is warranted. © 2014 Editrice Gastroenterologica Italiana S.r.l.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.