Recent data expanded the concept that inflammation is a critical component of tumor progression. The tumor microenvironment is an indispensable participant in the neoplastic process, fostering proliferation, survival and migration. Tumor cells co-opted signalling molecules of the innate immune system, such as selectins, chemokines and their receptors for invasion, migration and metastasis. In this respect, Virus-induced tumors, like Papillomavirus-induced Squamous Cell Carcinomas (SCC), could represent a paradigmatic example of interplay between inflammatory responses and malignant transformation. To establish a tumorigenic role of inflammatory mediators in HPV+ SCC, we analyzed by real time RT-PCR the expression of inflammatory cytokines, chemokines and related molecules in HPV+ carcinoma cell lines, HPV- SCC (C33A) and in human foreskin keratinocytes transduced by E6 and E7 derived from mucosal HPV-16 or cutaneous HPV-38 genotypes. We also tested the effect of the IFN-β on the levels of these pro-inflammatory mediators. In HPV+ SCC the level of both IL-1β and IL6 mRNAs were augmented if compared to HPV- SCC. Interestingly, levels of cytokines in supernantants, tested by CBA assay, revealed that despite huge increase in mRNA levels, these cells didn’t secrete IL-1β, whereas IL-6 appeared to be released by HPV+ SCC.
Pro-inflammatory cytokines analysis in HPV-positive cancer cells / Mangino, Giorgio; Zangrillo, MARIA SIMONA; M. V., Chiantore; Iuliano, Marco; M., Severa; G., Fiorucci; E. M., Coccia; Romeo, Giovanna. - (2014). (Intervento presentato al convegno Interferon fundamentals 2014, from molecular mechanisms to human diseases tenutosi a Rome nel 20/02/2014).
Pro-inflammatory cytokines analysis in HPV-positive cancer cells
MANGINO, GIORGIO;ZANGRILLO, MARIA SIMONA;IULIANO, MARCO;ROMEO, Giovanna
2014
Abstract
Recent data expanded the concept that inflammation is a critical component of tumor progression. The tumor microenvironment is an indispensable participant in the neoplastic process, fostering proliferation, survival and migration. Tumor cells co-opted signalling molecules of the innate immune system, such as selectins, chemokines and their receptors for invasion, migration and metastasis. In this respect, Virus-induced tumors, like Papillomavirus-induced Squamous Cell Carcinomas (SCC), could represent a paradigmatic example of interplay between inflammatory responses and malignant transformation. To establish a tumorigenic role of inflammatory mediators in HPV+ SCC, we analyzed by real time RT-PCR the expression of inflammatory cytokines, chemokines and related molecules in HPV+ carcinoma cell lines, HPV- SCC (C33A) and in human foreskin keratinocytes transduced by E6 and E7 derived from mucosal HPV-16 or cutaneous HPV-38 genotypes. We also tested the effect of the IFN-β on the levels of these pro-inflammatory mediators. In HPV+ SCC the level of both IL-1β and IL6 mRNAs were augmented if compared to HPV- SCC. Interestingly, levels of cytokines in supernantants, tested by CBA assay, revealed that despite huge increase in mRNA levels, these cells didn’t secrete IL-1β, whereas IL-6 appeared to be released by HPV+ SCC.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
