Conflicting data are reported on pro- or anti-inflammatory activity of bovine lactoferrin (bLf) in different cell models as phagocytes or epithelial cell lines infected by bacteria. Here we evaluated the bLf effect on epithelial models mimicking two human pathologies characterized by inflammation and infection with specific bacterial species. Primary bronchial epithelium from a cystic fibrosis (CF) patient and differentiated intestinal epithelial cells were infected with Pseudomonas aeruginosa LESB58 isolated from a CF patient and Adherent-Invasive Escherichia coli LF82 isolated from a Crohn's disease patient. Surprisingly, bLf significantly reduced the intracellular bacterial survival, but differently modulated the inflammatory response. These data lead us to hypothesize that bLf differentially acts depending on the epithelial model and infecting pathogen. To verify this hypothesis, we explored whether bLf could modulate ferroportin (Fpn), the only known cellular iron exporter from cells, that, by lowering the intracellular iron level, determines a non permissive environment for intracellular pathogens. Here, for the first time, we describe the bLf ability to up-regulate Fpn protein in infected epithelial models. Our data suggest that the mechanism underlying the bLf modulating activity on inflammatory response in epithelial cells is complex and the bLf involvement in modulating cellular iron homeostasis should be taken into account. © 2014 Springer Science+Business Media New York.

Lactoferrin differently modulates the inflammatory response in epithelial models mimicking human inflammatory and infectious diseases / Frioni, Alessandra; Conte, Maria Pia; Cutone, Antimo; Longhi, Catia; Giovanni, Musci; BONACCORSI DI PATTI, Maria Carmela; D. I., Maria Carmela Bonaccorsi Patti; M. C. B., Di Patti; Natalizi, Tiziana; Marazzato, Massimiliano; Patrizia, Puddu; Paesano, Rosalba; Valenti, Piera; Berlutti, Francesca; Lepanto, MARIA STEFANIA. - In: BIOMETALS. - ISSN 0966-0844. - ELETTRONICO. - 27:5(2014), pp. 843-856. [10.1007/s10534-014-9740-9]

Lactoferrin differently modulates the inflammatory response in epithelial models mimicking human inflammatory and infectious diseases

FRIONI, ALESSANDRA;CONTE, Maria Pia;CUTONE, ANTIMO;LONGHI, Catia;BONACCORSI DI PATTI, Maria Carmela;NATALIZI, TIZIANA;Marazzato, Massimiliano;PAESANO, Rosalba;VALENTI, PIERA;BERLUTTI, Francesca;LEPANTO, MARIA STEFANIA
2014

Abstract

Conflicting data are reported on pro- or anti-inflammatory activity of bovine lactoferrin (bLf) in different cell models as phagocytes or epithelial cell lines infected by bacteria. Here we evaluated the bLf effect on epithelial models mimicking two human pathologies characterized by inflammation and infection with specific bacterial species. Primary bronchial epithelium from a cystic fibrosis (CF) patient and differentiated intestinal epithelial cells were infected with Pseudomonas aeruginosa LESB58 isolated from a CF patient and Adherent-Invasive Escherichia coli LF82 isolated from a Crohn's disease patient. Surprisingly, bLf significantly reduced the intracellular bacterial survival, but differently modulated the inflammatory response. These data lead us to hypothesize that bLf differentially acts depending on the epithelial model and infecting pathogen. To verify this hypothesis, we explored whether bLf could modulate ferroportin (Fpn), the only known cellular iron exporter from cells, that, by lowering the intracellular iron level, determines a non permissive environment for intracellular pathogens. Here, for the first time, we describe the bLf ability to up-regulate Fpn protein in infected epithelial models. Our data suggest that the mechanism underlying the bLf modulating activity on inflammatory response in epithelial cells is complex and the bLf involvement in modulating cellular iron homeostasis should be taken into account. © 2014 Springer Science+Business Media New York.
2014
cystic fibrosis; ferroportin; inflammation; lactoferrin; infection; chron's disease
01 Pubblicazione su rivista::01a Articolo in rivista
Lactoferrin differently modulates the inflammatory response in epithelial models mimicking human inflammatory and infectious diseases / Frioni, Alessandra; Conte, Maria Pia; Cutone, Antimo; Longhi, Catia; Giovanni, Musci; BONACCORSI DI PATTI, Maria Carmela; D. I., Maria Carmela Bonaccorsi Patti; M. C. B., Di Patti; Natalizi, Tiziana; Marazzato, Massimiliano; Patrizia, Puddu; Paesano, Rosalba; Valenti, Piera; Berlutti, Francesca; Lepanto, MARIA STEFANIA. - In: BIOMETALS. - ISSN 0966-0844. - ELETTRONICO. - 27:5(2014), pp. 843-856. [10.1007/s10534-014-9740-9]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/557503
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 36
  • Scopus 61
  • ???jsp.display-item.citation.isi??? 57
social impact