The availability of an efficient plant regeneration system is a key starting point for developing in vitro procedures to culture and transform all Cucumis melo L. genotypes. Previous studies showed that the regeneration efficiency is strongly influenced by both genotype and medium. The aim of this work was to set up a good protocol for plant regeneration to be used in gene silencing approaches. We chose six melon genotypes, belonging to the var. cantalupensis, for their resistance (the double-haploid, DH, lines NAD, L6 and the cv. Isabelle) and susceptibility (the cvs. Charentais-T, Vedrantais and the DH L2) to Fusarium oxysporum f. sp. melonis (FOM) race 1,2, the most aggressive pathogen of melon. Cotyledon explants from 6-days seedlings were cultured on MS medium added with different combinations of BAP (1.0, 1.2, 1.3 mg/l) and IAA (1.0·10-2, 1.2·10-2, 1.3·10-2 mg/l). After 5 weeks of culture the number of shoots/explant ranked from 0.4 (L6) to 4.1 (Charentais-T), from 0.3 (L6) to 4.4 (Charentais-T) and from 0.1 (L6) to 2.4 (Vedrantais) when they grew into the combinations BAP 1.0-IAA 1.0·10-2 mg/l, BAP 1.2-IAA 1.2·10-2 mg/l and BAP 1.3-IAA 1.3·10-2 mg/l, respectively. Moreover the plant regeneration rate varied from 24% (Charentais-T, Isabelle) to 85% (L6), from 20% (L2) to 66% (NAD) and from 10% (Charentais-T) to 50% (L6) by using the same media. The efficacy and specificity of this protocol show that it can be used for the gene silencing to study candidate resistance genes to FOM
An efficient protocol for in vitro plant regeneration from cotyledon explants of different melon genotypes (Cucumis melo L. var. cantalupensis) / Sebastiani, MARIA SILVIA; N., Ficcadenti. - STAMPA. - (2013), p. p. 129. (Intervento presentato al convegno Young Researchers in Life Sciences conference tenutosi a Ecole Nationale de Chimie de Paris, Paris nel 21/24-05-2013).
An efficient protocol for in vitro plant regeneration from cotyledon explants of different melon genotypes (Cucumis melo L. var. cantalupensis).
SEBASTIANI, MARIA SILVIA;
2013
Abstract
The availability of an efficient plant regeneration system is a key starting point for developing in vitro procedures to culture and transform all Cucumis melo L. genotypes. Previous studies showed that the regeneration efficiency is strongly influenced by both genotype and medium. The aim of this work was to set up a good protocol for plant regeneration to be used in gene silencing approaches. We chose six melon genotypes, belonging to the var. cantalupensis, for their resistance (the double-haploid, DH, lines NAD, L6 and the cv. Isabelle) and susceptibility (the cvs. Charentais-T, Vedrantais and the DH L2) to Fusarium oxysporum f. sp. melonis (FOM) race 1,2, the most aggressive pathogen of melon. Cotyledon explants from 6-days seedlings were cultured on MS medium added with different combinations of BAP (1.0, 1.2, 1.3 mg/l) and IAA (1.0·10-2, 1.2·10-2, 1.3·10-2 mg/l). After 5 weeks of culture the number of shoots/explant ranked from 0.4 (L6) to 4.1 (Charentais-T), from 0.3 (L6) to 4.4 (Charentais-T) and from 0.1 (L6) to 2.4 (Vedrantais) when they grew into the combinations BAP 1.0-IAA 1.0·10-2 mg/l, BAP 1.2-IAA 1.2·10-2 mg/l and BAP 1.3-IAA 1.3·10-2 mg/l, respectively. Moreover the plant regeneration rate varied from 24% (Charentais-T, Isabelle) to 85% (L6), from 20% (L2) to 66% (NAD) and from 10% (Charentais-T) to 50% (L6) by using the same media. The efficacy and specificity of this protocol show that it can be used for the gene silencing to study candidate resistance genes to FOMI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.