BACKGROUND: Studies conducted in the prepubertal period showed that biomarkers of oxidative stress decreased with increasing age in normocholesterolemic children (NC), and, conversely, they are persistently high in hypercholesterolemic children (HC). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is the most important cellular source of reactive oxygen species. No data have been reported concerning the behavior of age-related oxidative stress generated by NOX2, the catalytic subunit of NADPH oxidase, in children. METHODS: We performed a cross-sectional study comparing oxidized low-density lipoprotein (ox-LDL), as a marker of oxidative stress, and NOX2 activity, as assessed by blood levels of soluble NOX2-derived peptide (sNOX2-dp), in a population of 250 children, induding 125 NC and 125 HC. RESULTS: HC had higher sNOX2-dp (25.8 +/- 16.1 pg/ml) and ox-LDL (23.6 +/- 14.4 U/I) levels as compared with NC (15.4 +/- 10.1 and 11.6 +/- 6.4 pg/ml, respectively; P < 0.001). Multiple linear regression analysis showed that ox-LDL and total cholesterol were the only independent predictive variables associated with sNOX2-dp. In NC, ox-LDL and sNOX2-dp significantly decreased from the first to the second quintile of age. In HC, ox-LDL and sNOX2-dp levels did not show significant differences among quintiles of age. CONCLUSION: This study suggests that NOX2 contributes to generating ox-LDL during the early phase of life in NC and to the persistent high oxidative stress in the prepubertal period of HC.

Early increase of NOX2-derived oxidative stress in children: relationship with age / Loffredo, Lorenzo; Pignatelli, Pasquale; Martino, Francesco; Carnevale, Roberto; Bartimoccia, Simona; Catasca, Elisa; Chiara, Colantoni; Cristina, Zanoni; Perri, Ludovica; Violi, Francesco. - In: PEDIATRIC RESEARCH. - ISSN 0031-3998. - STAMPA. - 73:6(2013), pp. 788-793. [10.1038/pr.2013.55]

Early increase of NOX2-derived oxidative stress in children: relationship with age

LOFFREDO, Lorenzo;PIGNATELLI, Pasquale;MARTINO, Francesco;CARNEVALE, Roberto;BARTIMOCCIA, SIMONA;CATASCA, ELISA;PERRI, LUDOVICA;VIOLI, Francesco
2013

Abstract

BACKGROUND: Studies conducted in the prepubertal period showed that biomarkers of oxidative stress decreased with increasing age in normocholesterolemic children (NC), and, conversely, they are persistently high in hypercholesterolemic children (HC). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is the most important cellular source of reactive oxygen species. No data have been reported concerning the behavior of age-related oxidative stress generated by NOX2, the catalytic subunit of NADPH oxidase, in children. METHODS: We performed a cross-sectional study comparing oxidized low-density lipoprotein (ox-LDL), as a marker of oxidative stress, and NOX2 activity, as assessed by blood levels of soluble NOX2-derived peptide (sNOX2-dp), in a population of 250 children, induding 125 NC and 125 HC. RESULTS: HC had higher sNOX2-dp (25.8 +/- 16.1 pg/ml) and ox-LDL (23.6 +/- 14.4 U/I) levels as compared with NC (15.4 +/- 10.1 and 11.6 +/- 6.4 pg/ml, respectively; P < 0.001). Multiple linear regression analysis showed that ox-LDL and total cholesterol were the only independent predictive variables associated with sNOX2-dp. In NC, ox-LDL and sNOX2-dp significantly decreased from the first to the second quintile of age. In HC, ox-LDL and sNOX2-dp levels did not show significant differences among quintiles of age. CONCLUSION: This study suggests that NOX2 contributes to generating ox-LDL during the early phase of life in NC and to the persistent high oxidative stress in the prepubertal period of HC.
2013
01 Pubblicazione su rivista::01a Articolo in rivista
Early increase of NOX2-derived oxidative stress in children: relationship with age / Loffredo, Lorenzo; Pignatelli, Pasquale; Martino, Francesco; Carnevale, Roberto; Bartimoccia, Simona; Catasca, Elisa; Chiara, Colantoni; Cristina, Zanoni; Perri, Ludovica; Violi, Francesco. - In: PEDIATRIC RESEARCH. - ISSN 0031-3998. - STAMPA. - 73:6(2013), pp. 788-793. [10.1038/pr.2013.55]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/530174
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
social impact