The aim of this study was to individuate, by bioassay-guided fractionation, promising antifungal fractions and/or constituents from Hypericum perforatum subsp. angustifolium in vitro roots. Treatments with chitosan, O-carboxymethylchitosan (CMC) and its derivatives were used to improve xanthone production in the roots. The bioassay-guided fractionation of CMC-treated roots led to the individuation of an ethyl acetate fraction, containing the highest amount of xanthones (6.8%) and showing the best antifungal activity with minimal inhibitory concentration (MIC) values of 53.82, 14.18, and 36.52 mu g/ml, against Candida spp., Cryptococcus neoformans and dermatophytes, respectively. From this fraction the prenylated xanthone, biyouxanthone D has been isolated and represented the 44.59% of all xanthones detected. For the first time in the present paper biyouxanthone D has been found in H. perforatum roots and tested against C neoformans, dermatophytes, and Candida species. The xanthone showed the greatest antifungal activity against C neoformans and dermatophytes, with MIC values of 20.16, 22.63 mu g/ml. In conclusion, the results obtained in the present study demonstrated that CMC-treated Hpa in vitro root extracts represent a tool for the obtainment of promising candidates for further pharmacological and clinical studies. (c) 2013 Elsevier Masson SAS. All rights reserved.
Bioassay-guided fractionation of extracts from Hypericum perforatum in vitro roots treated with carboxymethylchitosans and determination of antifungal activity against human fungal pathogens / Tocci, Noemi; D'Auria, Felicia Diodata; Simonetti, Giovanna; Panella, Simona; Palamara, ANNA TERESA; Aline, Debrassi; Antonio Rodrigues Clovis, ; Valdir Filho Cechinel, ; Clovis Antonio Rodrigues, ; Cechinel, V.; Valdir Cechinel Filho, ; Sciubba, Fabio; Pasqua, Gabriella. - In: PLANT PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 0981-9428. - STAMPA. - 70:(2013), pp. 342-347. [10.1016/j.plaphy.2013.05.046]
Bioassay-guided fractionation of extracts from Hypericum perforatum in vitro roots treated with carboxymethylchitosans and determination of antifungal activity against human fungal pathogens
TOCCI, Noemi;Felicia Diodata D'Auria;SIMONETTI, Giovanna;PANELLA, SIMONA;PALAMARA, ANNA TERESA;SCIUBBA, FABIO;PASQUA, Gabriella
2013
Abstract
The aim of this study was to individuate, by bioassay-guided fractionation, promising antifungal fractions and/or constituents from Hypericum perforatum subsp. angustifolium in vitro roots. Treatments with chitosan, O-carboxymethylchitosan (CMC) and its derivatives were used to improve xanthone production in the roots. The bioassay-guided fractionation of CMC-treated roots led to the individuation of an ethyl acetate fraction, containing the highest amount of xanthones (6.8%) and showing the best antifungal activity with minimal inhibitory concentration (MIC) values of 53.82, 14.18, and 36.52 mu g/ml, against Candida spp., Cryptococcus neoformans and dermatophytes, respectively. From this fraction the prenylated xanthone, biyouxanthone D has been isolated and represented the 44.59% of all xanthones detected. For the first time in the present paper biyouxanthone D has been found in H. perforatum roots and tested against C neoformans, dermatophytes, and Candida species. The xanthone showed the greatest antifungal activity against C neoformans and dermatophytes, with MIC values of 20.16, 22.63 mu g/ml. In conclusion, the results obtained in the present study demonstrated that CMC-treated Hpa in vitro root extracts represent a tool for the obtainment of promising candidates for further pharmacological and clinical studies. (c) 2013 Elsevier Masson SAS. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
