The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs.

Usutu virus growth in human cell lines: induction of and sensitivity to type I and III interferons / Scagnolari, Carolina; Caputo, Beniamino; Trombetti, Simona; Cacciotti, Giulia; A., Solda'; Spano, Lucia; Villari, Paolo; DELLA TORRE, Alessandra; N., Nowotny; Antonelli, Guido. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - STAMPA. - 94:Pt 4(2013), pp. 789-795. [10.1099/vir.0.046433-0]

Usutu virus growth in human cell lines: induction of and sensitivity to type I and III interferons

SCAGNOLARI, CAROLINA;CAPUTO, BENIAMINO;TROMBETTI, SIMONA;CACCIOTTI, GIULIA;SPANO, LUCIA;VILLARI, Paolo;DELLA TORRE, Alessandra;ANTONELLI, Guido
2013

Abstract

The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs.
2013
01 Pubblicazione su rivista::01a Articolo in rivista
Usutu virus growth in human cell lines: induction of and sensitivity to type I and III interferons / Scagnolari, Carolina; Caputo, Beniamino; Trombetti, Simona; Cacciotti, Giulia; A., Solda'; Spano, Lucia; Villari, Paolo; DELLA TORRE, Alessandra; N., Nowotny; Antonelli, Guido. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - STAMPA. - 94:Pt 4(2013), pp. 789-795. [10.1099/vir.0.046433-0]
File allegati a questo prodotto
File Dimensione Formato  
GJV.full.pdf

accesso aperto

Tipologia: Abstract
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 770.55 kB
Formato Adobe PDF
770.55 kB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/508811
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 13
social impact