A surprising and unexpected biomineralization process was observed during toxicological assessment of carbon nanoparticles on Paracentrotus lividus (sea urchin) pluteus larvae. The larvae activate a process of defense against external material, by incorporating the nanoparticles into microstructures of aragonite similarly to pearl oysters. Aiming at a better understanding of this phenomenon, the larvae were exposed to increasing concentrations of carbon nanoparticles and the biomineralization products were analyzed by electron microscopy, x-ray diffraction and Raman spectroscopy. In order to evaluate the possible influence of Sp-CyP-1 expression on this biomineralization process by larvae, analyses of gene expression (Sp-CyP-1) and calcein labeling were performed. Overall, we report experimental evidence about the capability of carbon nanoparticles to induce an increment of Sp-CyP-1 expression with the consequent activation of a biomineralization process leading to the production of a new pearl-like biomaterial never previously observed in sea urchins.

High ordered biomineralization induced by carbon nanoparticles in the sea urchin Paracentrotus lividus / Daniela, Manno; Elisabetta, Carata; Bernadetta A., Tenuzzo; Elisa, Panzarini; Alessandro, Buccolieri; E., Filippo; Rossi, Marco; Antonio, Serra; Luciana, Dini. - In: NANOTECHNOLOGY. - ISSN 0957-4484. - STAMPA. - 23:49(2012), p. 495104. [10.1088/0957-4484/23/49/495104]

High ordered biomineralization induced by carbon nanoparticles in the sea urchin Paracentrotus lividus

ROSSI, Marco;Luciana Dini
2012

Abstract

A surprising and unexpected biomineralization process was observed during toxicological assessment of carbon nanoparticles on Paracentrotus lividus (sea urchin) pluteus larvae. The larvae activate a process of defense against external material, by incorporating the nanoparticles into microstructures of aragonite similarly to pearl oysters. Aiming at a better understanding of this phenomenon, the larvae were exposed to increasing concentrations of carbon nanoparticles and the biomineralization products were analyzed by electron microscopy, x-ray diffraction and Raman spectroscopy. In order to evaluate the possible influence of Sp-CyP-1 expression on this biomineralization process by larvae, analyses of gene expression (Sp-CyP-1) and calcein labeling were performed. Overall, we report experimental evidence about the capability of carbon nanoparticles to induce an increment of Sp-CyP-1 expression with the consequent activation of a biomineralization process leading to the production of a new pearl-like biomaterial never previously observed in sea urchins.
2012
01 Pubblicazione su rivista::01a Articolo in rivista
High ordered biomineralization induced by carbon nanoparticles in the sea urchin Paracentrotus lividus / Daniela, Manno; Elisabetta, Carata; Bernadetta A., Tenuzzo; Elisa, Panzarini; Alessandro, Buccolieri; E., Filippo; Rossi, Marco; Antonio, Serra; Luciana, Dini. - In: NANOTECHNOLOGY. - ISSN 0957-4484. - STAMPA. - 23:49(2012), p. 495104. [10.1088/0957-4484/23/49/495104]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/502574
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