This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved.
Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor / Antiochia, Riccarda; Vinci, Giuliana; Lo, Gorton. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 140:4(2013), pp. 742-747. [10.1016/j.foodchem.2012.11.023]
Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor
ANTIOCHIA, RICCARDA;VINCI, Giuliana;
2013
Abstract
This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.