Recently, it was reported that, in several cell lines, dexamethasone, a synthetic glucocorticoid, modulates the sphingolipid metab. and the intracellular sphingosine levels. It was suggested that its effects could be mediated by the prodn. of sphingosine from membrane sphingomyelin. The present study examd. the effect of dexamethasone on Epstein-Barr virus transformed B lymphocytes on lipid compn. and metab. In particular sphingosine levels by 31P and 1H NMR spectroscopy, high performance liq. chromatog. and dexamethasone effects on membrane structure by fluorimetry were investigated. Furthermore, dexamethasone effects on cellular proliferation and IgM secretion were evaluated.
Biochemical and biophysical effects of dexamethasone on human lymphoblastoid cell lines, studied by phosphorus-31 and proton NMR spectroscopy and fluorimetry / R., Ricciolini; Miccheli, Alfredo; Piccolella, Enza; Tomassini, Alberta; Manetti, Cesare; DI COCCO, Maria Enrica; Conti, Filippo. - In: BULLETIN OF MAGNETIC RESONANCE. - ISSN 0163-559X. - STAMPA. - 15:3(1993), pp. 165-168.
Biochemical and biophysical effects of dexamethasone on human lymphoblastoid cell lines, studied by phosphorus-31 and proton NMR spectroscopy and fluorimetry
MICCHELI, Alfredo;PICCOLELLA, Enza;TOMASSINI, Alberta;MANETTI, Cesare;DI COCCO, Maria Enrica;CONTI, Filippo
1993
Abstract
Recently, it was reported that, in several cell lines, dexamethasone, a synthetic glucocorticoid, modulates the sphingolipid metab. and the intracellular sphingosine levels. It was suggested that its effects could be mediated by the prodn. of sphingosine from membrane sphingomyelin. The present study examd. the effect of dexamethasone on Epstein-Barr virus transformed B lymphocytes on lipid compn. and metab. In particular sphingosine levels by 31P and 1H NMR spectroscopy, high performance liq. chromatog. and dexamethasone effects on membrane structure by fluorimetry were investigated. Furthermore, dexamethasone effects on cellular proliferation and IgM secretion were evaluated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
