Recently, it was reported that, in several cell lines, dexamethasone, a synthetic glucocorticoid, modulates the sphingolipid metab. and the intracellular sphingosine levels. It was suggested that its effects could be mediated by the prodn. of sphingosine from membrane sphingomyelin. The present study examd. the effect of dexamethasone on Epstein-Barr virus transformed B lymphocytes on lipid compn. and metab. In particular sphingosine levels by 31P and 1H NMR spectroscopy, high performance liq. chromatog. and dexamethasone effects on membrane structure by fluorimetry were investigated. Furthermore, dexamethasone effects on cellular proliferation and IgM secretion were evaluated.

Biochemical and biophysical effects of dexamethasone on human lymphoblastoid cell lines, studied by phosphorus-31 and proton NMR spectroscopy and fluorimetry

MICCHELI, Alfredo;PICCOLELLA, Enza;TOMASSINI, Alberta;MANETTI, Cesare;DI COCCO, Maria Enrica;CONTI, Filippo
1993

Abstract

Recently, it was reported that, in several cell lines, dexamethasone, a synthetic glucocorticoid, modulates the sphingolipid metab. and the intracellular sphingosine levels. It was suggested that its effects could be mediated by the prodn. of sphingosine from membrane sphingomyelin. The present study examd. the effect of dexamethasone on Epstein-Barr virus transformed B lymphocytes on lipid compn. and metab. In particular sphingosine levels by 31P and 1H NMR spectroscopy, high performance liq. chromatog. and dexamethasone effects on membrane structure by fluorimetry were investigated. Furthermore, dexamethasone effects on cellular proliferation and IgM secretion were evaluated.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/470596
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