Biogel P2 gel chromatography of ultrafiltrate from chronic uremic patients leads to better resolution of the compounds present than so far reported in the literature. Reproducibility is good. Toxicity, evaluated by cell cultures, was detected in only one chromatographic fraction. Further purification of this fraction with HPLC on a RP18 column revealed two toxic fractions, to the better defined of which a polyol structure was ascribed.
Evaluation of blood toxicity in chronic uremia by an improved chromatographic method / Politi, Laura; A. R., D'Angelo; M., Caramia; Molinaro, Mario; Nicoletti, Rosario; Cerulli, Nicola; M., Moriggi; Scandurra, Roberto. - In: CLINICAL AND EXPERIMENTAL DIALYSIS AND APHERESIS.. - ISSN 0276-5497. - 5:3(1981), pp. 277-284.
Evaluation of blood toxicity in chronic uremia by an improved chromatographic method.
POLITI, Laura;MOLINARO, Mario;NICOLETTI, Rosario;CERULLI, Nicola;SCANDURRA, Roberto
1981
Abstract
Biogel P2 gel chromatography of ultrafiltrate from chronic uremic patients leads to better resolution of the compounds present than so far reported in the literature. Reproducibility is good. Toxicity, evaluated by cell cultures, was detected in only one chromatographic fraction. Further purification of this fraction with HPLC on a RP18 column revealed two toxic fractions, to the better defined of which a polyol structure was ascribed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
