The aim of this study was to assess how surface topography can induce osteoblast differentiation in mesenchymal stem cells by analyzing the expression levels of bone related genes and mesenchymal stem cells marker. Thirty disk-shaped, commercially pure Grade 2 titanium samples (10 x 2 mm) with 3 different surface topographies (DENTSPLY-Friadent GmbH, Mannheim, Germany) were used in the present study: 10 Ti machined disks (control), 10 Ti sandblasted and acid etched disks (DPS) and 10 sandblasted and acid etched disks at high temperature (Plus). Samples were processed for real time Reverse Transcription-Polymerase Chain Reaction (RT–PCR) analysis. By comparing machined and Plus disks quantitative real-time RT–PCR showed a significant reduction of the bone related genes osteocalcin (BGLAP) and osteoblast transcriptional factor (RUNX2). The comparison between sandblasted and Plus disks showed a slight induction of all the genes examined (RUNX2, ALPL, COL1A1, COL3A1, ENG, FOSL1, SPP1, and SP7); only the expression of BGLAP remained stable. The present study demonstrated that implant surface topography affects osteoblast gene expression. Indeed, Plus surface produces an effect on PB-hMSCs in the late differentiation stages. The obtained results contribute to the understanding of the molecular mechanism of implant osseointegration, the molecular events related to the differentiation of stem cells into osteoblasts and as a model for comparing dental implants with different surface topography.
In vitro analysis with human bone marrow stem cells on titanium disks with different surface topographies / Manzon, Licia; Vittoria, Perrotti; Annalisa, Palmieri; Agnese, Pellati; Marco, Degidi; Adriano, Piattelli; Francesco, Carinci. - STAMPA. - (2012), pp. 96-96. (Intervento presentato al convegno 27 annual meeting of the Accademy of Osteointegration. Tecnology to practice tenutosi a Phenix nel 1-3 Marzo 2012).
In vitro analysis with human bone marrow stem cells on titanium disks with different surface topographies
MANZON, Licia;
2012
Abstract
The aim of this study was to assess how surface topography can induce osteoblast differentiation in mesenchymal stem cells by analyzing the expression levels of bone related genes and mesenchymal stem cells marker. Thirty disk-shaped, commercially pure Grade 2 titanium samples (10 x 2 mm) with 3 different surface topographies (DENTSPLY-Friadent GmbH, Mannheim, Germany) were used in the present study: 10 Ti machined disks (control), 10 Ti sandblasted and acid etched disks (DPS) and 10 sandblasted and acid etched disks at high temperature (Plus). Samples were processed for real time Reverse Transcription-Polymerase Chain Reaction (RT–PCR) analysis. By comparing machined and Plus disks quantitative real-time RT–PCR showed a significant reduction of the bone related genes osteocalcin (BGLAP) and osteoblast transcriptional factor (RUNX2). The comparison between sandblasted and Plus disks showed a slight induction of all the genes examined (RUNX2, ALPL, COL1A1, COL3A1, ENG, FOSL1, SPP1, and SP7); only the expression of BGLAP remained stable. The present study demonstrated that implant surface topography affects osteoblast gene expression. Indeed, Plus surface produces an effect on PB-hMSCs in the late differentiation stages. The obtained results contribute to the understanding of the molecular mechanism of implant osseointegration, the molecular events related to the differentiation of stem cells into osteoblasts and as a model for comparing dental implants with different surface topography.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
