Argonaute are a conserved class of proteins central to the microRNA pathway. We have highlighted a novel and non-redundant function of Ago1 versus Ago2; the two core factors of the miRNA-associated RISC complex. Stable overexpression of Ago1 in neuroblastoma cells causes the cell cycle to slow down, a decrease in cellular motility and a stronger apoptotic response upon UV irradiation. These effects, together with a significant increase in p53 levels, suggest that Ago1 may act as a tumor-suppressor factor, a function also supported by GEO Profiles microarrays that inversely correlate Ago1 expression levels with cell proliferation rates. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Ago1 and Ago2 differentially affect cell proliferation, motility and apoptosis when overexpressed in SH-SY5Y neuroblastoma cells / Chiara, Parisi; Corinna, Giorgi; Enrico Maria, Batassa; Laura, Braccini; Giovanna, Maresca; Igea, D'Agnano; Caputo, Viviana; A., Salvatore; Flavia, Pietrolati; Cogoni, Carlo; Catalanotto, Caterina. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 585:19(2011), pp. 2965-2971. [10.1016/j.febslet.2011.08.003]
Ago1 and Ago2 differentially affect cell proliferation, motility and apoptosis when overexpressed in SH-SY5Y neuroblastoma cells
CAPUTO, VIVIANA;COGONI, Carlo;CATALANOTTO, Caterina
2011
Abstract
Argonaute are a conserved class of proteins central to the microRNA pathway. We have highlighted a novel and non-redundant function of Ago1 versus Ago2; the two core factors of the miRNA-associated RISC complex. Stable overexpression of Ago1 in neuroblastoma cells causes the cell cycle to slow down, a decrease in cellular motility and a stronger apoptotic response upon UV irradiation. These effects, together with a significant increase in p53 levels, suggest that Ago1 may act as a tumor-suppressor factor, a function also supported by GEO Profiles microarrays that inversely correlate Ago1 expression levels with cell proliferation rates. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
