One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only 1 strain with "low discrimination" whereas the VITEK2 gave 12. In any case, the two biochemical systems showed good reliability compared to SS-PCR.

Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical system and species-specific PCR / Giordano, Alessandra; Magni, Annarita; Trancassini, Maria; Varesi, Paola; Turner, R.; Mancini, Carlo. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 64(1):(2006), pp. 135-138. [10.1016/j.mimet.2005.04.020]

Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical system and species-specific PCR

GIORDANO, Alessandra;MAGNI, Annarita;TRANCASSINI, Maria;VARESI, PAOLA;MANCINI, Carlo
2006

Abstract

One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only 1 strain with "low discrimination" whereas the VITEK2 gave 12. In any case, the two biochemical systems showed good reliability compared to SS-PCR.
2006
01 Pubblicazione su rivista::01a Articolo in rivista
Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical system and species-specific PCR / Giordano, Alessandra; Magni, Annarita; Trancassini, Maria; Varesi, Paola; Turner, R.; Mancini, Carlo. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 64(1):(2006), pp. 135-138. [10.1016/j.mimet.2005.04.020]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/365472
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