Immunosuppressive status in solid organ transplant recipients is often related to the reactivation of Human Cytomegalovirus (HCMV) infection that remains one of the major causes of morbidity and mortality. Therefore, the early detection of HCMV followed by infection monitoring is important to institute prompt and appropriate treatment. In recent years good results have been obtained by HCMV DNA amplification methods; qualitative and quantitative approaches have shown good sensitivity and specificity, but they often require post-PCR manipulation that adds time to the analysis and may lead to contamination problems. Recently, Real Time PCR (RT-PCR) has been proposed in HCMV DNA analysis as a valid method for its good sensitivity and rapidity. In the present study, twenty-five solid organ transplant recipients were analyzed for HCMV diagnosis; 60 peripheral blood leukocytes and 120 plasma samples were tested by RT-PCR and the results compared to those obtained by a qualitative Nested PCR and a quantitative DNA enzyme immunoassay.

Application of Real Time PCR in post transplant monitoring of cytomegalovirus infection: comparison with other diagnostic approaches / Gaeta, Aurelia; Nazzari, Cristina; Verzaro, Simona; Latte, Maria Cristina; G., Fabri; Mancini, Carlo. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - STAMPA. - 29:3(2006), pp. 185-192.

Application of Real Time PCR in post transplant monitoring of cytomegalovirus infection: comparison with other diagnostic approaches

GAETA, Aurelia;NAZZARI, Cristina;VERZARO, Simona;LATTE, Maria Cristina;MANCINI, Carlo
2006

Abstract

Immunosuppressive status in solid organ transplant recipients is often related to the reactivation of Human Cytomegalovirus (HCMV) infection that remains one of the major causes of morbidity and mortality. Therefore, the early detection of HCMV followed by infection monitoring is important to institute prompt and appropriate treatment. In recent years good results have been obtained by HCMV DNA amplification methods; qualitative and quantitative approaches have shown good sensitivity and specificity, but they often require post-PCR manipulation that adds time to the analysis and may lead to contamination problems. Recently, Real Time PCR (RT-PCR) has been proposed in HCMV DNA analysis as a valid method for its good sensitivity and rapidity. In the present study, twenty-five solid organ transplant recipients were analyzed for HCMV diagnosis; 60 peripheral blood leukocytes and 120 plasma samples were tested by RT-PCR and the results compared to those obtained by a qualitative Nested PCR and a quantitative DNA enzyme immunoassay.
2006
cytomegalovirus; real time pcr; transplant
01 Pubblicazione su rivista::01a Articolo in rivista
Application of Real Time PCR in post transplant monitoring of cytomegalovirus infection: comparison with other diagnostic approaches / Gaeta, Aurelia; Nazzari, Cristina; Verzaro, Simona; Latte, Maria Cristina; G., Fabri; Mancini, Carlo. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - STAMPA. - 29:3(2006), pp. 185-192.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/364803
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