Metabolic profiling is a metabolomic approach that allows the characterization of metabolic phenotypes under specific set of conditions. In the present paper we investigated the metabolism of sparse and high density cultures in relation to different cell growth phases. Changes in the metabolome were evaluated by using H-1-NMR spectroscopy, correlation map and Multivariate Data Analysis on the net balances of metabolites in the medium. This approach allowed us to identify two different metabolic profiles in relation to the cell growth phases in subconfluence and confluence cultures. The results have been interpreted on the basis of patterns of correlations obtained in the two physiological cell states. Cells almost arrested in G0/G1 phase by contact dependent growth inhibition underwent changes in the channeling of amino acids utilization from synthetic to energetic purpose and in anaplerosis/cataplerosis regulation of the TCA cycle. (c) 2006 Elsevier B.V. All rights reserved.

NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis / Tomassini, Alberta; Miccheli, Alfredo; DI CLEMENTE, Roberta; Mariacristina, Valerio; Bizzarri, Mariano; Conti, Filippo; Coluccia, Pier Paolo. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - 1760:11(2006), pp. 1723-1731. [10.1016/j.bbagen.2006.09.009]

NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis

TOMASSINI, Alberta;MICCHELI, Alfredo;DI CLEMENTE, ROBERTA;BIZZARRI, Mariano;CONTI, Filippo;COLUCCIA, Pier Paolo
2006

Abstract

Metabolic profiling is a metabolomic approach that allows the characterization of metabolic phenotypes under specific set of conditions. In the present paper we investigated the metabolism of sparse and high density cultures in relation to different cell growth phases. Changes in the metabolome were evaluated by using H-1-NMR spectroscopy, correlation map and Multivariate Data Analysis on the net balances of metabolites in the medium. This approach allowed us to identify two different metabolic profiles in relation to the cell growth phases in subconfluence and confluence cultures. The results have been interpreted on the basis of patterns of correlations obtained in the two physiological cell states. Cells almost arrested in G0/G1 phase by contact dependent growth inhibition underwent changes in the channeling of amino acids utilization from synthetic to energetic purpose and in anaplerosis/cataplerosis regulation of the TCA cycle. (c) 2006 Elsevier B.V. All rights reserved.
2006
1h nmr; amino acid metabolism; cancer cell; h-1 nmr; hepg2; metabolomics
01 Pubblicazione su rivista::01a Articolo in rivista
NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis / Tomassini, Alberta; Miccheli, Alfredo; DI CLEMENTE, Roberta; Mariacristina, Valerio; Bizzarri, Mariano; Conti, Filippo; Coluccia, Pier Paolo. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - 1760:11(2006), pp. 1723-1731. [10.1016/j.bbagen.2006.09.009]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/364618
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