An automated on-line solid-phase extraction-liquid chromatography- electrospray tandem mass spectrometry (SPE-LC-ESI-MS/MS) method was developed for the determination of ochratoxin A (OTA) in alcoholic beverages. Mean recoveries for wine and beer were, respectively, 75 and 82%. Detection was achieved in negative ionization with a Q TRAP mass spectrometer operating in multiple-reaction monitoring (MRM) mode or enhanced product ion (EPI) mode, using the third quadrupole as linear ion trap. The MRM mode turned out to be more sensitive; the method allowed accurate determination of OTA in the range of 0.01-25 ng ml-1 using external calibration. Within-day and between-day relative standard deviation percentages were <6.2 and <9.1%, respectively. In EPI mode, fragmentation spectra at the limit of quantification (0.03 ng mL-1) and good linearity could be obtained. Application of the method (MRM mode) to the analysis of several wine and beer samples purchased in local stores revealed OTA levels in the ranges of 0.03-1.44 ng mL -1 for wines and 0.02-0.14 ng mL-1 for beers.

Automated On-line Solid-Phase Extraction-Liquid Chromatography-Electrospray Tandem Mass Spectrometry Method for the Determination of Ochratoxin in Wine and Beer / Bacaloni, Alessandro; Cavaliere, Chiara; A., Faberi; E., Pastorini; Samperi, Roberto; Lagana', Aldo. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - STAMPA. - 53:14(2005), pp. 5518-5525. [10.1021/jf050254+]

Automated On-line Solid-Phase Extraction-Liquid Chromatography-Electrospray Tandem Mass Spectrometry Method for the Determination of Ochratoxin in Wine and Beer

BACALONI, Alessandro;CAVALIERE, CHIARA;SAMPERI, Roberto;LAGANA', Aldo
2005

Abstract

An automated on-line solid-phase extraction-liquid chromatography- electrospray tandem mass spectrometry (SPE-LC-ESI-MS/MS) method was developed for the determination of ochratoxin A (OTA) in alcoholic beverages. Mean recoveries for wine and beer were, respectively, 75 and 82%. Detection was achieved in negative ionization with a Q TRAP mass spectrometer operating in multiple-reaction monitoring (MRM) mode or enhanced product ion (EPI) mode, using the third quadrupole as linear ion trap. The MRM mode turned out to be more sensitive; the method allowed accurate determination of OTA in the range of 0.01-25 ng ml-1 using external calibration. Within-day and between-day relative standard deviation percentages were <6.2 and <9.1%, respectively. In EPI mode, fragmentation spectra at the limit of quantification (0.03 ng mL-1) and good linearity could be obtained. Application of the method (MRM mode) to the analysis of several wine and beer samples purchased in local stores revealed OTA levels in the ranges of 0.03-1.44 ng mL -1 for wines and 0.02-0.14 ng mL-1 for beers.
ochratoxin A; food analysis; automated method; liquid chromatography; mass spectrometry; linear ion trap
01 Pubblicazione su rivista::01a Articolo in rivista
Automated On-line Solid-Phase Extraction-Liquid Chromatography-Electrospray Tandem Mass Spectrometry Method for the Determination of Ochratoxin in Wine and Beer / Bacaloni, Alessandro; Cavaliere, Chiara; A., Faberi; E., Pastorini; Samperi, Roberto; Lagana', Aldo. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - STAMPA. - 53:14(2005), pp. 5518-5525. [10.1021/jf050254+]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/359727
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