The influence of retinoic acid on the expression of a typical marker of hepatocyte differentiation, i.e. the asialoglycoprotein receptor, has been studied. Cultured hepatocytes, isolated from adult rats, a model of quiescent, mature cells and from 20-day-old fetuses, a model of proliferating and less differentiated cells, were used. The asialoglycoprotein receptor expression appears to be affected by retinoic acid during prenatal life; both mRNA level and protein amount increased in fetal hepatocytes, but no modification has been found in adult cells, suggesting a regulative effect of retinoic acid during prenatal life, acting at transcriptional and:or translational level. Surprisingly, the receptor binding activity of adult hepatocytes is decreased after retinoic acid treatment, indicating a possible further modulation by this molecule on receptor activity at the post-translational level. © 2001 Elsevier Science Ireland Ltd. All rights reserved.
Retinoic acid modulates the asialoglycoprotein receptor expression in cultured fetal rat hepatocytes / Laura, Falasca; Angelina, Felici; Mara, Massimi; Dini, Luciana; Conti, Laura. - In: MECHANISMS OF AGEING AND DEVELOPMENT. - ISSN 0047-6374. - STAMPA. - 122:1(2001), pp. 31-39. [10.1016/s0047-6374(00)00174-3]
Retinoic acid modulates the asialoglycoprotein receptor expression in cultured fetal rat hepatocytes
Luciana Dini;CONTI, Laura
2001
Abstract
The influence of retinoic acid on the expression of a typical marker of hepatocyte differentiation, i.e. the asialoglycoprotein receptor, has been studied. Cultured hepatocytes, isolated from adult rats, a model of quiescent, mature cells and from 20-day-old fetuses, a model of proliferating and less differentiated cells, were used. The asialoglycoprotein receptor expression appears to be affected by retinoic acid during prenatal life; both mRNA level and protein amount increased in fetal hepatocytes, but no modification has been found in adult cells, suggesting a regulative effect of retinoic acid during prenatal life, acting at transcriptional and:or translational level. Surprisingly, the receptor binding activity of adult hepatocytes is decreased after retinoic acid treatment, indicating a possible further modulation by this molecule on receptor activity at the post-translational level. © 2001 Elsevier Science Ireland Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
