We studied the effect of continuous medium flow on the viability and structural organization of hepatocytes high density entrapped in alginate gel beads in the first few hours after isolation. The metabolic energy status of the entrapped cells, monitored in vivo by P-31 NMR spectroscopy, was stable during the experimental time and a physiological redox ratio was reached after the first three hours of culture. The morphological analysis revealed that the entrapped hepatocytes placed in a fixed-bed bioreactor under continuous flow showed a polyhedrical shape with numerous microvilli on cell surface and reconstituted tight junctions as well as bile canalicular structures, closely resembling those present in the liver. These results suggest that continuous flow allows the culture of hepatocytes at very high cell density within a matrix without loss of viability and accelerates cellular tissue reconstruction at very short times after isolation. This type of culture could represent a very useful model for physiological and toxicological studies as well as a promising approach toward the development of a bioartificial hybrid support device in acute liver failure.

Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes / Miccheli, Alfredo; Tomassini, Alberta; Capuani, Giorgio; DI COCCO, Maria Enrica; E., Sartori; L., Falasca; Conti, Laura; Manetti, Cesare; Conti, Filippo. - In: CYTOTECHNOLOGY. - ISSN 0920-9069. - STAMPA. - 32:3(2000), pp. 219-228. [10.1023/A:1008134005529]

Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes

MICCHELI, Alfredo;TOMASSINI, Alberta;CAPUANI, Giorgio;DI COCCO, Maria Enrica;CONTI, Laura;MANETTI, Cesare;CONTI, Filippo
2000

Abstract

We studied the effect of continuous medium flow on the viability and structural organization of hepatocytes high density entrapped in alginate gel beads in the first few hours after isolation. The metabolic energy status of the entrapped cells, monitored in vivo by P-31 NMR spectroscopy, was stable during the experimental time and a physiological redox ratio was reached after the first three hours of culture. The morphological analysis revealed that the entrapped hepatocytes placed in a fixed-bed bioreactor under continuous flow showed a polyhedrical shape with numerous microvilli on cell surface and reconstituted tight junctions as well as bile canalicular structures, closely resembling those present in the liver. These results suggest that continuous flow allows the culture of hepatocytes at very high cell density within a matrix without loss of viability and accelerates cellular tissue reconstruction at very short times after isolation. This type of culture could represent a very useful model for physiological and toxicological studies as well as a promising approach toward the development of a bioartificial hybrid support device in acute liver failure.
2000
alginate; bioreactor; energy metabolism; hepatocytes; microscopy; NMR; RAT HEPATOCYTES; EXTRACELLULAR-MATRIX; BIOARTIFICIAL LIVER; CALCIUM ALGINATE; CELL; CULTURE; BEADS; MAINTENANCE; P-31-NMR; CONFIGURATION
01 Pubblicazione su rivista::01a Articolo in rivista
Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes / Miccheli, Alfredo; Tomassini, Alberta; Capuani, Giorgio; DI COCCO, Maria Enrica; E., Sartori; L., Falasca; Conti, Laura; Manetti, Cesare; Conti, Filippo. - In: CYTOTECHNOLOGY. - ISSN 0920-9069. - STAMPA. - 32:3(2000), pp. 219-228. [10.1023/A:1008134005529]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/250472
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 9
social impact