The murine immune response to Escherichia coli O6:K- alone or pre-exposed to 0.1 x MIC of aztreonam was investigated. Relative to mice immunized with untreated bacteria, mice immunized with antibiotic-treated microorganisms presented a significantly enhanced protection towards a challenge of 100 x LD50 of viable E. coli O6:K-. Previous injection of 0.1 mL of serum drawn from mice immunized with treated and untreated bacteria protected non-immunized mice towards a challenge of 10 x LD50 of viable E. coli O6:K-. Serum from mice immunized with treated bacteria also protected non-immunized mice towards a lethal challenge of E. coli O111. The antiserum contained high titre of IgG antibodies that cross-reacted with lipopolysaccharide isolated from smooth and rough Gram-negative bacteria. Immunoblotting showed additional bands of reactivity to the untreated E. coli O6:K-. Immunization with antibiotic-treated bacteria led to the production of type specific and cross reactive antibodies that protected animals against viable homologous and heterologous lethal challenges.
REACTIVITY AND PROTECTIVE CAPACITY OF A POLYCLONAL ANTISERUM DERIVED FROM MICE IMMUNIZED WITH ANTIBIOTIC EXPOSED ESCHERICHIA-COLI / Raponi, Giammarco; Lun, Maria Teresa; Lorino, Giulia; Nazzari, Cristina; Gaeta, Aurelia; Mancini, Carlo; Filadoro, Francesco. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - STAMPA. - 31:(1993), pp. 117-128. [10.1093/jac/31.1.117]
REACTIVITY AND PROTECTIVE CAPACITY OF A POLYCLONAL ANTISERUM DERIVED FROM MICE IMMUNIZED WITH ANTIBIOTIC EXPOSED ESCHERICHIA-COLI
RAPONI, Giammarco;LUN, Maria Teresa;LORINO, Giulia;NAZZARI, Cristina;GAETA, Aurelia;MANCINI, Carlo;FILADORO, Francesco
1993
Abstract
The murine immune response to Escherichia coli O6:K- alone or pre-exposed to 0.1 x MIC of aztreonam was investigated. Relative to mice immunized with untreated bacteria, mice immunized with antibiotic-treated microorganisms presented a significantly enhanced protection towards a challenge of 100 x LD50 of viable E. coli O6:K-. Previous injection of 0.1 mL of serum drawn from mice immunized with treated and untreated bacteria protected non-immunized mice towards a challenge of 10 x LD50 of viable E. coli O6:K-. Serum from mice immunized with treated bacteria also protected non-immunized mice towards a lethal challenge of E. coli O111. The antiserum contained high titre of IgG antibodies that cross-reacted with lipopolysaccharide isolated from smooth and rough Gram-negative bacteria. Immunoblotting showed additional bands of reactivity to the untreated E. coli O6:K-. Immunization with antibiotic-treated bacteria led to the production of type specific and cross reactive antibodies that protected animals against viable homologous and heterologous lethal challenges.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.