Reverse-transcriptase polymerase chain reaction has been used to analyze the expression of 2 latent genes (EBNA-1 and LMP-1) and one replicative gene (BZLF-1) of Epstein-Barr virus in mononuclear cells from bone marrow and peripheral blood of healthy donors. EBV-gene transcription was detected in 8 out of 15 bone-marrow samples. Among these, 5 allowed the detection of latency-associated transcripts in the absence of BZLF-1 expression, Only one sample showed positivity for expression of both latent and lytic genes. in 2 cases, BZLF-1 was the only transcript detected. In peripheral blood, 4 out of 7 samples showed evidence of EBNA-1 transcription; LMP-1 was expressed in 5 samples, and in 2 cases concomitant expression of EBNA-1 and BZLF-1 was detected, These results provide a direct demonstration by RT-PCR of EBV gene transcription in bone-marrow-resident viral infected cells and suggest, in contrast to previous studies on peripheral blood, that LMP-1 and BZLF-1 are frequently transcribed also in absence of EBV-related disease. The heterogeneous viral gene expression found makes it difficult to define a pattern of viral latency in vivo which coincides with that described for lymphoblastoid or Burkitt's-lymphoma cell lines at different stages of differentiation

Transcription of latent and replicative Epstein-Barr virus genes in bone marrow and peripheral blood mononuclear cells of healthy donors / Gonnella, Roberta; Angeloni, Antonio; Calogero, Antonella; Farina, Antonella; Santarelli, Roberta; Gentile, Giuseppe; Arcese, W; Martino, P; Mandelli, Franco; Frati, Luigi; Faggioni, Alberto; Ragona, Giuseppe. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - STAMPA. - 70:(1997), pp. 524-529. [10.1002/(SICI)1097-0215(19970304)70:5<524::AID-IJC6>3.0.CO;2]

Transcription of latent and replicative Epstein-Barr virus genes in bone marrow and peripheral blood mononuclear cells of healthy donors.

GONNELLA, ROBERTA;ANGELONI, Antonio;CALOGERO, ANTONELLA;FARINA, Antonella;SANTARELLI, Roberta;GENTILE, Giuseppe;MANDELLI, Franco;FRATI, Luigi;FAGGIONI, Alberto;RAGONA, Giuseppe
1997

Abstract

Reverse-transcriptase polymerase chain reaction has been used to analyze the expression of 2 latent genes (EBNA-1 and LMP-1) and one replicative gene (BZLF-1) of Epstein-Barr virus in mononuclear cells from bone marrow and peripheral blood of healthy donors. EBV-gene transcription was detected in 8 out of 15 bone-marrow samples. Among these, 5 allowed the detection of latency-associated transcripts in the absence of BZLF-1 expression, Only one sample showed positivity for expression of both latent and lytic genes. in 2 cases, BZLF-1 was the only transcript detected. In peripheral blood, 4 out of 7 samples showed evidence of EBNA-1 transcription; LMP-1 was expressed in 5 samples, and in 2 cases concomitant expression of EBNA-1 and BZLF-1 was detected, These results provide a direct demonstration by RT-PCR of EBV gene transcription in bone-marrow-resident viral infected cells and suggest, in contrast to previous studies on peripheral blood, that LMP-1 and BZLF-1 are frequently transcribed also in absence of EBV-related disease. The heterogeneous viral gene expression found makes it difficult to define a pattern of viral latency in vivo which coincides with that described for lymphoblastoid or Burkitt's-lymphoma cell lines at different stages of differentiation
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/242020
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