The interaction between bovine serum albumin (BSA) and sodium taurodeoxycholate (NaTDC), a component of the bile in mammals, have been investigated in a wide range of experimental conditions and for several protein to surfactant ratios. The solution region has been investigated by surface tension σ, freezing point depression ΔT, as well as by integral heat of dilution data ΔHi,dil. The corresponding properties of NaTDC in aqueous solutions have been investigated too. Surfactant binding onto the protein is presumably controlled by a delicate balance of hydrophobic and electrostatic contributions, responsible for the adduct stability, but does not give rise to precipitation or coacervate formation. This behaviour is in line with current knowledge on the solution behaviour of BSA-TDC complexes occurring in hepatic and gallbladder bile. © 2004 Elsevier B.V. All rights reserved.
Interactions between bovine serum albumin and sodium taurodeoxycholate: Thermodynamic properties / Aida C., Palacios; Antonelli, Marta Letizia; LA MESA, Camillo. - In: THERMOCHIMICA ACTA. - ISSN 0040-6031. - STAMPA. - 418:1-2(2004), pp. 69-77. [10.1016/j.tca.2003.11.043]
Interactions between bovine serum albumin and sodium taurodeoxycholate: Thermodynamic properties
ANTONELLI, Marta Letizia;LA MESA, Camillo
2004
Abstract
The interaction between bovine serum albumin (BSA) and sodium taurodeoxycholate (NaTDC), a component of the bile in mammals, have been investigated in a wide range of experimental conditions and for several protein to surfactant ratios. The solution region has been investigated by surface tension σ, freezing point depression ΔT, as well as by integral heat of dilution data ΔHi,dil. The corresponding properties of NaTDC in aqueous solutions have been investigated too. Surfactant binding onto the protein is presumably controlled by a delicate balance of hydrophobic and electrostatic contributions, responsible for the adduct stability, but does not give rise to precipitation or coacervate formation. This behaviour is in line with current knowledge on the solution behaviour of BSA-TDC complexes occurring in hepatic and gallbladder bile. © 2004 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
