In this study, a method for assessing the occurrence of trace amounts of 12 representative estrogenic compounds in sewage and surface waters was developed. The selected substances were the phytoestrogens daidzein, genistein and biochanin A, the alkylphenols bisphenol A and 4-nonylphenol, the natural hormones 17beta-estradiol, estrone, estriol and the synthetic hormone 17alpha-ethynylestradiol and the mycoestrogens zearalenone and two of its metabolites (alpha-zearalanol and beta-zearalanol). The procedure consists in solid phase extraction (SPE) performed with OASIS cartridges followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Recoveries were all above 80% for each analyzed aqueous matrices. The developed method was applied to verify the occurrence of endocrine disrupters in environmental samples of sewage influents and effluents of an Italian STP. Phytoestrogens were present in effluents at concentrations ranging from 3 to 83 ng/l, whereas the levels detected for alkylphenols were in the range 13-36 ng/l for bisphenol A and up to 1 mug/l for nonylphenol. Estrogens and zeranols were determined at levels below 30 ng/l. Analysis of a river (Tiber) receiving effluent waters revealed high quantities of bisphenol A (15-29 ng/l) and nonylphenol (up to 1.2 mug/l), whereas the presence of all the other compounds were at levels of few ng/l.

Analytical Methodologies for the Determination and Occurrence of Endocrin Disrupting Chemicals in Sewage Treatment Plants and Natural Waters / Lagana', Aldo; Bacaloni, Alessandro; DE LEVA, Ilaria; Faberi, A; Fago, Giovanna; Marino, Aldo. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 501(1):1(2004), pp. 79-88. [10.1016/j.aca.2003.09.020]

Analytical Methodologies for the Determination and Occurrence of Endocrin Disrupting Chemicals in Sewage Treatment Plants and Natural Waters

LAGANA', Aldo;BACALONI, Alessandro;DE LEVA, ilaria;FAGO, Giovanna;MARINO, Aldo
2004

Abstract

In this study, a method for assessing the occurrence of trace amounts of 12 representative estrogenic compounds in sewage and surface waters was developed. The selected substances were the phytoestrogens daidzein, genistein and biochanin A, the alkylphenols bisphenol A and 4-nonylphenol, the natural hormones 17beta-estradiol, estrone, estriol and the synthetic hormone 17alpha-ethynylestradiol and the mycoestrogens zearalenone and two of its metabolites (alpha-zearalanol and beta-zearalanol). The procedure consists in solid phase extraction (SPE) performed with OASIS cartridges followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Recoveries were all above 80% for each analyzed aqueous matrices. The developed method was applied to verify the occurrence of endocrine disrupters in environmental samples of sewage influents and effluents of an Italian STP. Phytoestrogens were present in effluents at concentrations ranging from 3 to 83 ng/l, whereas the levels detected for alkylphenols were in the range 13-36 ng/l for bisphenol A and up to 1 mug/l for nonylphenol. Estrogens and zeranols were determined at levels below 30 ng/l. Analysis of a river (Tiber) receiving effluent waters revealed high quantities of bisphenol A (15-29 ng/l) and nonylphenol (up to 1.2 mug/l), whereas the presence of all the other compounds were at levels of few ng/l.
2004
EDCs; water analysis; STPs; liquid chromatography tandem mass spectrometry; SPE
01 Pubblicazione su rivista::01a Articolo in rivista
Analytical Methodologies for the Determination and Occurrence of Endocrin Disrupting Chemicals in Sewage Treatment Plants and Natural Waters / Lagana', Aldo; Bacaloni, Alessandro; DE LEVA, Ilaria; Faberi, A; Fago, Giovanna; Marino, Aldo. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 501(1):1(2004), pp. 79-88. [10.1016/j.aca.2003.09.020]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/236429
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