Here, we explore various strategies and report our recent results about the realization of optical biosensors based on vibrational spectroscopy. We spectroscopically characterize the analytes. The object of our propose is Spike protein (subunit S1) of three variants of SARSCoV- 2 virus. We prove the potential to discriminate the three proteins from their IR amide I band (1600-1710 cm-1) and to exploit them as a biomarker. A structural investigation is also deepened, putting aside experimental results with a computational simulation analysis, leading the spectral differences back to changes in variants S1 proteins conformational structure. Following, various approaches involving nanostructured platforms and chemical treatments have been developed. Preliminary results are illustrated. These constitute the first steps for the realization of new optical device in the field of airborne biosensing.
First step toward the development of an optical biosensor based on vibrational spectroscopy for airborne pathogens detection / Mancini, Tiziana; D’Arco, Annalisa; Mosetti, Rosanna; Macis, Salvatore; Tranfo, Giovanna; Della Ventura, Giancarlo; Marcelli, Augusto; Lupi, Stefano. - (2024). (Intervento presentato al convegno Rome Technopole Spoke 6 - International Young Researcher Workshop tenutosi a Rome).
First step toward the development of an optical biosensor based on vibrational spectroscopy for airborne pathogens detection
Tiziana Mancini
;Annalisa D’Arco;Rosanna Mosetti;Salvatore Macis;Stefano Lupi
2024
Abstract
Here, we explore various strategies and report our recent results about the realization of optical biosensors based on vibrational spectroscopy. We spectroscopically characterize the analytes. The object of our propose is Spike protein (subunit S1) of three variants of SARSCoV- 2 virus. We prove the potential to discriminate the three proteins from their IR amide I band (1600-1710 cm-1) and to exploit them as a biomarker. A structural investigation is also deepened, putting aside experimental results with a computational simulation analysis, leading the spectral differences back to changes in variants S1 proteins conformational structure. Following, various approaches involving nanostructured platforms and chemical treatments have been developed. Preliminary results are illustrated. These constitute the first steps for the realization of new optical device in the field of airborne biosensing.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.