The synthesis of a novel functional biomaterial for wound healing treatment wascarried out by adopting a free-radical grafting procedure in aqueous media. With this aim,ciprofloxacin (CFX) was covalently incorporated into collagen (T1C) chains employing anascorbic acid/hydrogen peroxide redox pair as biocompatible initiator system. The covalentinsertion of CFX in the polymeric chains was confirmed by FT-IR and UV analyses, whilean antibacterial assay demonstrated the activity of the synthesized conjugate againstStaphylococcus aureus and Escherichia coli, microorganisms that commonly infectwounds. A catechin blended conjugate was also tested in order to evaluate the ability toinfluence fibroblast cell growth. The observed antibacterial activity and stimulation of fibroblast growth support the applicability of CFX-T1C conjugate in wound treatmentencouraging the healing process.
Ciprofloxacin-collagen conjugate in the wound healingtreatment / Puoci, F; Piangiolino, C; Givigliano, F; Parisi, Oi; Cassano, R; Trombino, S; Curcio, M; Iemma, F; Cirillo, G; Spizzirri, Ug; Restuccia, D; Muzzalupo, R; Picci, N. - In: JOURNAL OF FUNCTIONAL BIOMATERIALS. - ISSN 2079-4983. - 3:(2012), pp. 361-371. [10.3390/jfb3020361]
Ciprofloxacin-collagen conjugate in the wound healingtreatment
Trombino S;Restuccia D;
2012
Abstract
The synthesis of a novel functional biomaterial for wound healing treatment wascarried out by adopting a free-radical grafting procedure in aqueous media. With this aim,ciprofloxacin (CFX) was covalently incorporated into collagen (T1C) chains employing anascorbic acid/hydrogen peroxide redox pair as biocompatible initiator system. The covalentinsertion of CFX in the polymeric chains was confirmed by FT-IR and UV analyses, whilean antibacterial assay demonstrated the activity of the synthesized conjugate againstStaphylococcus aureus and Escherichia coli, microorganisms that commonly infectwounds. A catechin blended conjugate was also tested in order to evaluate the ability toinfluence fibroblast cell growth. The observed antibacterial activity and stimulation of fibroblast growth support the applicability of CFX-T1C conjugate in wound treatmentencouraging the healing process.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
