Biofilm formation and lipopolysaccharide (LPS) are implicated in the pathogenesis of gastrointestinal (GI) diseases caused by Gram-negative bacteria. Grape seeds, wine industry by-products, have antioxidant and antimicrobial activity. In the present study, the protective effect of procyanidin-rich grape seed extract (prGSE), from unfermented pomace of Vitis vinifera L. cv Bellone, on bacterial LPS-induced oxidative stress and epithelial barrier integrity damage has been studied in a model of Caco-2 cells. The prGSE was characterized at the molecular level using HPLC and NMR. The in vitro activity of prGSE against formation of biofilm of Salmonella enterica subsp. enterica serovar Typhimurium and Escherichia coli was investigated. In vivo, prGSE activity using infected Galleria mellonella larvae has been evaluated. The results show that the prGSE, if administered with LPS, can significantly reduce the LPS-induced permeability alteration. Moreover, the ability of the extract to prevent Reactive Oxygen Species (ROS) production induced by the LPS treatment of Caco-2 cells was demonstrated. prGSE inhibited the biofilm formation of E. coli and S. Typhimurium. In terms of in vivo activity, an increase in survival of infected G. mellonella larvae after treatment with prGSE was demonstrated. In conclusion, grape seed extracts could be used to reduce GI damage caused by bacterial endotoxin and biofilms of Gram-negative bacteria.

Protective effect of procyanidin-rich grape seed extract against Gram-negative virulence factors / Nicolosi, ROBERTA MARIA; Bonincontro, Graziana; Imperia, Elena; Badiali, Camilla; DE VITA, Daniela; Sciubba, Fabio; Dugo, Laura; Pier Luca Guarino, Michele; Altomare, Annamaria; Simonetti, Giovanna; Pasqua, Gabriella. - In: ANTIBIOTICS. - ISSN 2079-6382. - 12:(2023), pp. 1-20. [10.20944/preprints202307.2137.v1]

Protective effect of procyanidin-rich grape seed extract against Gram-negative virulence factors

Roberta Maria Nicolosi;Graziana Bonincontro;Camilla Badiali;Daniela De Vita;Fabio Sciubba;Giovanna Simonetti
;
Gabriella Pasqua
2023

Abstract

Biofilm formation and lipopolysaccharide (LPS) are implicated in the pathogenesis of gastrointestinal (GI) diseases caused by Gram-negative bacteria. Grape seeds, wine industry by-products, have antioxidant and antimicrobial activity. In the present study, the protective effect of procyanidin-rich grape seed extract (prGSE), from unfermented pomace of Vitis vinifera L. cv Bellone, on bacterial LPS-induced oxidative stress and epithelial barrier integrity damage has been studied in a model of Caco-2 cells. The prGSE was characterized at the molecular level using HPLC and NMR. The in vitro activity of prGSE against formation of biofilm of Salmonella enterica subsp. enterica serovar Typhimurium and Escherichia coli was investigated. In vivo, prGSE activity using infected Galleria mellonella larvae has been evaluated. The results show that the prGSE, if administered with LPS, can significantly reduce the LPS-induced permeability alteration. Moreover, the ability of the extract to prevent Reactive Oxygen Species (ROS) production induced by the LPS treatment of Caco-2 cells was demonstrated. prGSE inhibited the biofilm formation of E. coli and S. Typhimurium. In terms of in vivo activity, an increase in survival of infected G. mellonella larvae after treatment with prGSE was demonstrated. In conclusion, grape seed extracts could be used to reduce GI damage caused by bacterial endotoxin and biofilms of Gram-negative bacteria.
2023
grape seed extract; Vitis vinifera L.; anti-virulence factors; LPS; bacterial biofilm; Salmonella enterica subsp. enterica serovar Typhimurium; Escherichia coli; Galleria mellonella; Caco-2
01 Pubblicazione su rivista::01a Articolo in rivista
Protective effect of procyanidin-rich grape seed extract against Gram-negative virulence factors / Nicolosi, ROBERTA MARIA; Bonincontro, Graziana; Imperia, Elena; Badiali, Camilla; DE VITA, Daniela; Sciubba, Fabio; Dugo, Laura; Pier Luca Guarino, Michele; Altomare, Annamaria; Simonetti, Giovanna; Pasqua, Gabriella. - In: ANTIBIOTICS. - ISSN 2079-6382. - 12:(2023), pp. 1-20. [10.20944/preprints202307.2137.v1]
File allegati a questo prodotto
File Dimensione Formato  
Nicolosi_Protective-effect_2023.pdf

accesso aperto

Note: Articolo rivista
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 3.05 MB
Formato Adobe PDF
3.05 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1692695
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact