The global rise of single-use throw-away plastic products has elicited a massive increase in the nano/microplastics (N/MPLs) exposure burden in humans. Recently, it has been demonstrated that disposable period products may release N/MPLs with usage, which represents a potential threat to women's health which has not been scientifically addressed yet. By using polyethyl ene (PE) particles (200 nm to 9 & mu;m), we showed that acute exposure to a high concentration of N/MPLs induced cell toxicity in vaginal keratinocytes after effective cellular uptake, as viability and apoptosis data suggest, along with transmission electron microscopy (TEM) observations. The internalised N/MPLs altered the expression of junctional and adherence proteins and the organisation of the actin cortex, influencing the level of genes involved in oxidative stress signalling pathways and that of miRNAs related to epithelial barrier function. When the exposure to PE N/MPLs was discontinued or became chronic, cells were able to recover from the negative effects on viability and differentiation/proliferation gene expression in a few days. However, in all cases, PE N/MPL exposure prompted a sustained alteration of DNA methyltransferase and DNA demethylase expression, which might impact epigenetic regulation processes, leading to accelerated cell ageing and inflammation, or the occurrence of malignant transformation.

Assessing the Impact of Polyethylene Nano/Microplastic Exposure on Human Vaginal Keratinocytes / Pontecorvi, Paola; Ceccarelli, Simona; Cece, Fabrizio; Camero, Simona; Lotti, Lavinia Vittoria; Niccolai, Elena; Nannini, Giulia; Gerini, Giulia; Anastasiadou, Eleni; Sofia Scialis, Elena; Romano, Enrico; Venneri, MARY ANNA; Amedei, Amedeo; Angeloni, Antonio; Megiorni, Francesca; Marchese, Cinzia. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:14(2023), pp. 1-25. [10.3390/ijms241411379]

Assessing the Impact of Polyethylene Nano/Microplastic Exposure on Human Vaginal Keratinocytes

Paola Pontecorvi
Primo
;
Simona Ceccarelli;Simona Camero;Lavinia Vittoria Lotti;Giulia Gerini;Eleni Anastasiadou;Enrico Romano;Mary Anna Venneri;Amedeo Amedei;Antonio Angeloni;Francesca Megiorni;Cinzia Marchese
2023

Abstract

The global rise of single-use throw-away plastic products has elicited a massive increase in the nano/microplastics (N/MPLs) exposure burden in humans. Recently, it has been demonstrated that disposable period products may release N/MPLs with usage, which represents a potential threat to women's health which has not been scientifically addressed yet. By using polyethyl ene (PE) particles (200 nm to 9 & mu;m), we showed that acute exposure to a high concentration of N/MPLs induced cell toxicity in vaginal keratinocytes after effective cellular uptake, as viability and apoptosis data suggest, along with transmission electron microscopy (TEM) observations. The internalised N/MPLs altered the expression of junctional and adherence proteins and the organisation of the actin cortex, influencing the level of genes involved in oxidative stress signalling pathways and that of miRNAs related to epithelial barrier function. When the exposure to PE N/MPLs was discontinued or became chronic, cells were able to recover from the negative effects on viability and differentiation/proliferation gene expression in a few days. However, in all cases, PE N/MPL exposure prompted a sustained alteration of DNA methyltransferase and DNA demethylase expression, which might impact epigenetic regulation processes, leading to accelerated cell ageing and inflammation, or the occurrence of malignant transformation.
2023
microplastics; nano/microparticles uptake; nanoplastics; period products; polyethylene; vaginal keratinocytes
01 Pubblicazione su rivista::01a Articolo in rivista
Assessing the Impact of Polyethylene Nano/Microplastic Exposure on Human Vaginal Keratinocytes / Pontecorvi, Paola; Ceccarelli, Simona; Cece, Fabrizio; Camero, Simona; Lotti, Lavinia Vittoria; Niccolai, Elena; Nannini, Giulia; Gerini, Giulia; Anastasiadou, Eleni; Sofia Scialis, Elena; Romano, Enrico; Venneri, MARY ANNA; Amedei, Amedeo; Angeloni, Antonio; Megiorni, Francesca; Marchese, Cinzia. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:14(2023), pp. 1-25. [10.3390/ijms241411379]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1687094
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