Ultraviolet-C (UVC) has been used to cause virus inactivation. The virucidal activity of three UV light lamps [UVC high frequencies (HF), UVC+B LED and UVC+A LED] was evaluated against the enveloped feline coronavirus (FCoVII), a surrogate model of SARS-CoV-2, the enveloped vesicular stomatitis virus (VSV), and the naked encephalomyocarditis virus (EMCV). Virucidal assays were performed at different time points of UV-light exposure (i.e., 5, 30 minutes and 1, 6, and 8 hours), placing each virus 180 cm below the perpendicular irradiation of the lamp and 1 and 2 meters from the perpendicular axis. We found that the UVC HF lamp had virucidal effects (≥96.8% of virus inactivation) against FCoVII, VSV and EMCV after 5 minutes of irradiation at each distance analyzed. Moreover, the UVC+B LED lamp had the highest inhibitory effects on FCoVII and VSV infectivity (≥99% of virus inactivation) when these viruses were settled below the perpendicular axis of the lamp for 5 minutes. Conversely, the UVC+A LED lamp was the least effective, achieving ≥85.9% inactivation of enveloped RNA viruses after 8 hours of UV exposure. Overall, UV light lamps, and in particular UVC HF and UVC+B LED ones, had a rapid and strong virucidal activity against distinct RNA viruses, including coronaviruses.

In Vitro Virucidal Effects of Ultraviolet Light Prototypes on RNA viruses / Fracella, Matteo; Scordio, Mirko; Sorrentino, Leonardo; D'Auria, Alessandra; Ingenito, Raffaele; Gentile, Massimo; Pierangeli, Alessandra; Antonelli, Guido; Scagnolari, Carolina; Frasca, Federica. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 46:2(2023), pp. 161-169.

In Vitro Virucidal Effects of Ultraviolet Light Prototypes on RNA viruses

Fracella, Matteo;Scordio, Mirko;Sorrentino, Leonardo;D'Auria, Alessandra;Gentile, Massimo;Pierangeli, Alessandra;Antonelli, Guido;Scagnolari, Carolina;Frasca, Federica
2023

Abstract

Ultraviolet-C (UVC) has been used to cause virus inactivation. The virucidal activity of three UV light lamps [UVC high frequencies (HF), UVC+B LED and UVC+A LED] was evaluated against the enveloped feline coronavirus (FCoVII), a surrogate model of SARS-CoV-2, the enveloped vesicular stomatitis virus (VSV), and the naked encephalomyocarditis virus (EMCV). Virucidal assays were performed at different time points of UV-light exposure (i.e., 5, 30 minutes and 1, 6, and 8 hours), placing each virus 180 cm below the perpendicular irradiation of the lamp and 1 and 2 meters from the perpendicular axis. We found that the UVC HF lamp had virucidal effects (≥96.8% of virus inactivation) against FCoVII, VSV and EMCV after 5 minutes of irradiation at each distance analyzed. Moreover, the UVC+B LED lamp had the highest inhibitory effects on FCoVII and VSV infectivity (≥99% of virus inactivation) when these viruses were settled below the perpendicular axis of the lamp for 5 minutes. Conversely, the UVC+A LED lamp was the least effective, achieving ≥85.9% inactivation of enveloped RNA viruses after 8 hours of UV exposure. Overall, UV light lamps, and in particular UVC HF and UVC+B LED ones, had a rapid and strong virucidal activity against distinct RNA viruses, including coronaviruses.
2023
rna viruses; uva; uvb; uvc; coronavirus; disinfection; virucidal activity
01 Pubblicazione su rivista::01a Articolo in rivista
In Vitro Virucidal Effects of Ultraviolet Light Prototypes on RNA viruses / Fracella, Matteo; Scordio, Mirko; Sorrentino, Leonardo; D'Auria, Alessandra; Ingenito, Raffaele; Gentile, Massimo; Pierangeli, Alessandra; Antonelli, Guido; Scagnolari, Carolina; Frasca, Federica. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 46:2(2023), pp. 161-169.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1681572
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