Inflammatory bowel diseases (IBDs) are chronic and multifactorial inflammatory conditions of the colonic mucosa (ulcerative colitis), characterized by increased and unbalanced immune response to external stimuli. Garlic and its bioactive constituents were reported to exert various biological effects, including anti-inflammatory, antioxidant and immunomodulatory activities. We aimed to evaluate the protective effects of a hydroalcoholic (GHE) and a water (GWE) extract from a Sicilian variety of garlic, known as Nubia red garlic, on an ex vivo experimental model of ulcerative colitis, involving isolated LPS-treated mouse colon specimens. Both extracts were able to counteract LPS-induced cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-α, nuclear factor-kB (NF-kB), and interleukin (IL)-6 gene expression in mouse colon. Moreover, the same extracts inhibited prostaglandin (PG)E2, 8-iso-PGF2α, and increased the 5-hydroxyindoleacetic acid/serotonin ratio following treatment with LPS. In particular, GHE showed a better anti-inflammatory profile. The anti-inflammatory and antioxidant effects induced by both extracts could be related, at least partially, to their polyphenolic composition, with particular regards to catechin. Concluding, our results showed that GHE and GWE exhibited protective effects in colon, thus suggesting their potential use in the prevention and management of ulcerative colitis.

Anti-inflammatory and antioxidant effects induced by Allium sativum L. extracts on an ex vivo experimental model of ulcerative colitis / Recinella, Lucia; Gorica, Era; Chiavaroli, Annalisa; Fraschetti, Caterina; Filippi, Antonello; Cesa, Stefania; Cairone, Francesco; Martelli, Alma; Calderone, Vincenzo; Veschi, Serena; Lanuti, Paola; Cama, Alessandro; Orlando, Giustino; Ferrante, Claudio; Menghini, Luigi; Cristina Di Simone, Simonetta; Acquaviva, Alessandra; Loreta Libero, Maria; Nilofar, Nilofar; Brunetti, Luigi; Leone, Sheila. - In: FOODS. - ISSN 2304-8158. - 11:(2022), pp. 1-20. [10.3390/foods11223559]

Anti-inflammatory and antioxidant effects induced by Allium sativum L. extracts on an ex vivo experimental model of ulcerative colitis

Caterina Fraschetti;Antonello Filippi;Stefania Cesa;Francesco Cairone;Luigi Menghini;
2022

Abstract

Inflammatory bowel diseases (IBDs) are chronic and multifactorial inflammatory conditions of the colonic mucosa (ulcerative colitis), characterized by increased and unbalanced immune response to external stimuli. Garlic and its bioactive constituents were reported to exert various biological effects, including anti-inflammatory, antioxidant and immunomodulatory activities. We aimed to evaluate the protective effects of a hydroalcoholic (GHE) and a water (GWE) extract from a Sicilian variety of garlic, known as Nubia red garlic, on an ex vivo experimental model of ulcerative colitis, involving isolated LPS-treated mouse colon specimens. Both extracts were able to counteract LPS-induced cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-α, nuclear factor-kB (NF-kB), and interleukin (IL)-6 gene expression in mouse colon. Moreover, the same extracts inhibited prostaglandin (PG)E2, 8-iso-PGF2α, and increased the 5-hydroxyindoleacetic acid/serotonin ratio following treatment with LPS. In particular, GHE showed a better anti-inflammatory profile. The anti-inflammatory and antioxidant effects induced by both extracts could be related, at least partially, to their polyphenolic composition, with particular regards to catechin. Concluding, our results showed that GHE and GWE exhibited protective effects in colon, thus suggesting their potential use in the prevention and management of ulcerative colitis.
2022
garlic; colon; multimethodological evaluation; CIEL*a*b*; HS–SPME/GC–MS; apoptosis; inflammatory bowel disease
01 Pubblicazione su rivista::01a Articolo in rivista
Anti-inflammatory and antioxidant effects induced by Allium sativum L. extracts on an ex vivo experimental model of ulcerative colitis / Recinella, Lucia; Gorica, Era; Chiavaroli, Annalisa; Fraschetti, Caterina; Filippi, Antonello; Cesa, Stefania; Cairone, Francesco; Martelli, Alma; Calderone, Vincenzo; Veschi, Serena; Lanuti, Paola; Cama, Alessandro; Orlando, Giustino; Ferrante, Claudio; Menghini, Luigi; Cristina Di Simone, Simonetta; Acquaviva, Alessandra; Loreta Libero, Maria; Nilofar, Nilofar; Brunetti, Luigi; Leone, Sheila. - In: FOODS. - ISSN 2304-8158. - 11:(2022), pp. 1-20. [10.3390/foods11223559]
File allegati a questo prodotto
File Dimensione Formato  
Recinella_Anti-Inflammatory_2022.pdf

accesso aperto

Note: Articolo rivista
Tipologia: Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza: Creative commons
Dimensione 2.89 MB
Formato Adobe PDF
2.89 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1665120
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 4
social impact