In this work, we present a multifunctional Lab-on-Chip (LoC) platform based on hydrogenated amorphous silicon sensors suitable for a wide range of application in the fields of biochemical and food quality control analysis. The proposed system includes a LoC fabricated on a 5 cm × 5 cm glass substrate and a set of electronic boards for controlling the LoC functionalities. The presented Lab-on-Chip comprises light and temperature sensors, a thin film resistor acting as a heating source, and an optional thin film interferential filter suitable for fluorescence analysis. The developed electronics allows to control the thin film heater, a light source for fluorescence and absorption measurements, and the photosensors to acquire luminescent signals. All these modules are enclosed in a black metal box ensuring the portability of the whole platform. System performances have been evaluated in terms of sensor optical performances and thermal control achievements. For optical sensors, we have found a minimum number of detectable photons of 8 × 104 s−1·cm−2 at room temperature, 1.6 × 106 s−1·cm−2 in presence of fluorescence excitation source, and 2.4 × 106 s−1·cm−2 at 90 °C. From a thermal management point of view, we have obtained heating and cooling rates both equal to 2.2 °C/s, and a temperature sensor sensitivity of about 3 mV/°C even in presence of light. The achieved performances demonstrate the possibility to simultaneously use all integrated sensors and actuators, making promising the presented platform for a wide range of application fields.

Thin-Film-Based Multifunctional System for Optical Detection and Thermal Treatment of Biological Samples / Lovecchio, Nicola; Costantini, Francesca; Nascetti, Augusto; DE CESARE, Giampiero; Caputo, Domenico. - In: BIOSENSORS. - ISSN 2079-6374. - 12:11(2022), pp. 1-21. [10.3390/bios12110969]

Thin-Film-Based Multifunctional System for Optical Detection and Thermal Treatment of Biological Samples

NICOLA LOVECCHIO
;
Francesca Costantini;Augusto Nascetti;Giampiero de Cesare;Domenico Caputo
2022

Abstract

In this work, we present a multifunctional Lab-on-Chip (LoC) platform based on hydrogenated amorphous silicon sensors suitable for a wide range of application in the fields of biochemical and food quality control analysis. The proposed system includes a LoC fabricated on a 5 cm × 5 cm glass substrate and a set of electronic boards for controlling the LoC functionalities. The presented Lab-on-Chip comprises light and temperature sensors, a thin film resistor acting as a heating source, and an optional thin film interferential filter suitable for fluorescence analysis. The developed electronics allows to control the thin film heater, a light source for fluorescence and absorption measurements, and the photosensors to acquire luminescent signals. All these modules are enclosed in a black metal box ensuring the portability of the whole platform. System performances have been evaluated in terms of sensor optical performances and thermal control achievements. For optical sensors, we have found a minimum number of detectable photons of 8 × 104 s−1·cm−2 at room temperature, 1.6 × 106 s−1·cm−2 in presence of fluorescence excitation source, and 2.4 × 106 s−1·cm−2 at 90 °C. From a thermal management point of view, we have obtained heating and cooling rates both equal to 2.2 °C/s, and a temperature sensor sensitivity of about 3 mV/°C even in presence of light. The achieved performances demonstrate the possibility to simultaneously use all integrated sensors and actuators, making promising the presented platform for a wide range of application fields.
2022
Lab-on-Chip; amorphous silicon sensors; luminescence detection; fluorescence detection; temperature control system; portable multifunctional platform; DNA amplification system
01 Pubblicazione su rivista::01a Articolo in rivista
Thin-Film-Based Multifunctional System for Optical Detection and Thermal Treatment of Biological Samples / Lovecchio, Nicola; Costantini, Francesca; Nascetti, Augusto; DE CESARE, Giampiero; Caputo, Domenico. - In: BIOSENSORS. - ISSN 2079-6374. - 12:11(2022), pp. 1-21. [10.3390/bios12110969]
File allegati a questo prodotto
File Dimensione Formato  
Lovecchio_Thin-Film_2022.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 643.28 kB
Formato Adobe PDF
643.28 kB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1659041
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 3
social impact