Among noble metal nanoparticles, silver nanoparticles (AgNPs) represent suitable material in various a sensors, electronics, and nanomedicine applications. Another field in which silver nanoparticles are interesting to study is nanoagriculture, which deals with the development of engineered nanomaterials for agricultural applications of various kinds [1,2]. The aim of this study is the synthesis of AgNPs functionalized with a thiol ligand, with controlled size and surface charge. This research project belongs to the activities related to TANA Regione Lazio activities and will be used in mycorrhization process, i.e., a symbiotic relationship between fungi and roots of specific plants, that improve rhizosphere formation. Specifically, hydrophilic AgNPs in the 30-50 nm size range were synthesised via green synthesis procedure, using water as solvent and stabilized during their growth with two different functionalizing agents: a hydrophilic commercially available thiol, i.e. 3-mercapto-1-propansulfonate sodium salt (AgNPs-3MPS) and an hydrophilic synthetic polymer, i.e. poly(acrylic acid) (AgNPs-PAA) in order to compare the different stabilizing effect. The formation of AgNPs was confirmed by the presence of the localized surface plasmon band centred in the 400-450 nm wavelength region, for nanostructured silver. The presence of the ligands on the AgNPs surface was confirmed by infrared spectroscopy (FT-IR) and the size and spherical morphology was established by field-emission scanning electron microscopy (FE-SEM) at solid-state. Hydrodynamic size and stability in aqueous medium were evaluated by dynamic light scattering (DLS) and UV-vis for an interval of 0-30 days in the range of 5-50 g mL-1 and 1-0.2 mg mL-1 for AgNPs-3MPS and AgNPs-PAA, respectively. The stability of the two systems was assessed, opening perspectives on their use as helper in the growth of plants mycorrhized with Tuber melanosporum (black truffle) (Figure 1). [1] C. An et al., Nanobiotechnology 2022, 20, 1–19. [2] I. Schiesaro et al., Journal of Physical Chemistry C 2020, 124, 25975−25983.
Nanotechnology in agriculture: the role of silver nanoparticles in mycorrhization process / Mercurio, Martina; Cerra, Sara; Salamone, Tommaso A.; HAJAREH HAGHIGHI, Farid; Pagliarani, Sofia; Morales-Rodriguez, Carmen; Kuzminsky, Elena; Vannini, Andrea; Fratoddi, Ilaria. - (2022). (Intervento presentato al convegno XLVIII Italian Conference of Inorganic Chemistry tenutosi a Polo Didattico San Rossore 1938).
Nanotechnology in agriculture: the role of silver nanoparticles in mycorrhization process
Martina Mercurio;Sara Cerra;Tommaso A. Salamone;Farid Hajareh Haghighi;Ilaria Fratoddi
2022
Abstract
Among noble metal nanoparticles, silver nanoparticles (AgNPs) represent suitable material in various a sensors, electronics, and nanomedicine applications. Another field in which silver nanoparticles are interesting to study is nanoagriculture, which deals with the development of engineered nanomaterials for agricultural applications of various kinds [1,2]. The aim of this study is the synthesis of AgNPs functionalized with a thiol ligand, with controlled size and surface charge. This research project belongs to the activities related to TANA Regione Lazio activities and will be used in mycorrhization process, i.e., a symbiotic relationship between fungi and roots of specific plants, that improve rhizosphere formation. Specifically, hydrophilic AgNPs in the 30-50 nm size range were synthesised via green synthesis procedure, using water as solvent and stabilized during their growth with two different functionalizing agents: a hydrophilic commercially available thiol, i.e. 3-mercapto-1-propansulfonate sodium salt (AgNPs-3MPS) and an hydrophilic synthetic polymer, i.e. poly(acrylic acid) (AgNPs-PAA) in order to compare the different stabilizing effect. The formation of AgNPs was confirmed by the presence of the localized surface plasmon band centred in the 400-450 nm wavelength region, for nanostructured silver. The presence of the ligands on the AgNPs surface was confirmed by infrared spectroscopy (FT-IR) and the size and spherical morphology was established by field-emission scanning electron microscopy (FE-SEM) at solid-state. Hydrodynamic size and stability in aqueous medium were evaluated by dynamic light scattering (DLS) and UV-vis for an interval of 0-30 days in the range of 5-50 g mL-1 and 1-0.2 mg mL-1 for AgNPs-3MPS and AgNPs-PAA, respectively. The stability of the two systems was assessed, opening perspectives on their use as helper in the growth of plants mycorrhized with Tuber melanosporum (black truffle) (Figure 1). [1] C. An et al., Nanobiotechnology 2022, 20, 1–19. [2] I. Schiesaro et al., Journal of Physical Chemistry C 2020, 124, 25975−25983.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
