One of the main problems in the development of immunosensors is to overcome the complexity of binding antibodies (Abs) onto the sensor surface, thus leading to a loss of sensitivity. 1 In the last twenty years, preorganized macrocycles, i.e. calixarenes, were employed for the site-directed immobilization of Abs to promote the correct end-on orientation. 2 Recently, due to their configuration and unique molecular recognition properties, resorcinol-based cyclooligomes, namely resorcarenes, 3 turned out to be a good option for the construction of highly sensitive immunosensors. 4 In this project, resorc[4]arene architectures decorated with eight methoxy groups at the upper rim and substituted at the lower rim with different functional groups (i.e., 3-bromopropyloxy and 3-azidopropiloxy substituents) were rationally designed and synthesized to tailor their recognition properties towards the Fc portion of Abs and to covalently install the artificial linkers on the multi-walled carbon nanotubes (MWCNTs) surface. Different chemical methods including nucleophilic substitution, click chemistry, or direct cycloaddition via nitrene were exploited. The MWCNTs modified with the resorc[4]arenes were morphologically and electronically characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and X-ray photoemission spectroscopy (XPS) before the fabrication of modified electrodes. The latter were electrochemically characterized, and IgG Abs for the SARS-CoV-2 spike protein S1 (SPS1) were immobilized on their surface. The well-designed resorc[4]arene-functionalized MWCNTs-based immunosensors emerged as powerful systems for the development of more efficient immunosensing devices.

Resorc[4]arene-functionalized MWCNTs for the development of new highly sensitive electrochemical immunosensors / Cianfoni, Gabriele; Polli, Francesca; Elnahas, Rem; Ghirga, Francesca; Scaramuzzo, Francesca Anna; Cammarone, Silvia; Quaglio, Deborah; Calcaterra, Andrea; Mazzei, Franco; Zanoni, Robertino; Botta, Bruno. - (2022). ((Intervento presentato al convegno 1st International Supramolecular Chemistry Summer School tenutosi a Santa Margherita di Pula (CA).

Resorc[4]arene-functionalized MWCNTs for the development of new highly sensitive electrochemical immunosensors

Gabriele Cianfoni
;
Francesca Polli;Rem Elnahas;Francesca Ghirga;Francesca Scaramuzzo;Silvia Cammarone;Deborah Quaglio;Andrea Calcaterra;Franco Mazzei;Robertino Zanoni;Bruno Botta
2022

Abstract

One of the main problems in the development of immunosensors is to overcome the complexity of binding antibodies (Abs) onto the sensor surface, thus leading to a loss of sensitivity. 1 In the last twenty years, preorganized macrocycles, i.e. calixarenes, were employed for the site-directed immobilization of Abs to promote the correct end-on orientation. 2 Recently, due to their configuration and unique molecular recognition properties, resorcinol-based cyclooligomes, namely resorcarenes, 3 turned out to be a good option for the construction of highly sensitive immunosensors. 4 In this project, resorc[4]arene architectures decorated with eight methoxy groups at the upper rim and substituted at the lower rim with different functional groups (i.e., 3-bromopropyloxy and 3-azidopropiloxy substituents) were rationally designed and synthesized to tailor their recognition properties towards the Fc portion of Abs and to covalently install the artificial linkers on the multi-walled carbon nanotubes (MWCNTs) surface. Different chemical methods including nucleophilic substitution, click chemistry, or direct cycloaddition via nitrene were exploited. The MWCNTs modified with the resorc[4]arenes were morphologically and electronically characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and X-ray photoemission spectroscopy (XPS) before the fabrication of modified electrodes. The latter were electrochemically characterized, and IgG Abs for the SARS-CoV-2 spike protein S1 (SPS1) were immobilized on their surface. The well-designed resorc[4]arene-functionalized MWCNTs-based immunosensors emerged as powerful systems for the development of more efficient immunosensing devices.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/1645713
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